O-phosphoseryl-tRNA (Sec) selenium transferase (SEPSECS) Recombinant Protein | SEPSECS recombinant protein

Recombinant Human O-phosphoseryl-tRNA (Sec) selenium transferase (SEPSECS), partial

Cat. Num. AAA1265288

• Gene Names: SEPSECS; LP; SLA; PCH2D; SLA/LP
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: O-phosphoseryl-tRNA (Sec) selenium transferase (SEPSECS); Recombinant Human O-phosphoseryl-tRNA (Sec) selenium transferase (SEPSECS); partial; Liver-pancreas antigen ; LPSLA-p35SLA/LP autoantigen; Selenocysteine synthase ; Sec synthase; Selenocysteinyl-tRNA(Sec) synthase; Sep-tRNA:Sec-tRNA synthase ; SepSecSSoluble liver antigen ; SLAUGA suppressor tRNA-associated proteintRNA(Ser/Sec)-associated antigenic protein; SEPSECS recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 5-451aa, Partial
• Sequence: SFAAGERLVSPAYVRQGCEARRSHEHLIRLLLEKGKCPENGWDESTLELFLHELAIMDSNNFLGNCGVGEREGRVASALVARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLDIIKLAGVHTVANCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSMITAGFEPVVIENVLEGDELRTDLKAVEAKVQELGPDCILCIHSTTSCFAPRVPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGYKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLKTLDEHRDKAVTQLGSMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLFI

• Species: Homo sapiens (Human)
• Subcellular Location: Cytoplasm
• Protein Families: SepSecS family
• Tissue Specificity: Primarily expressed in liver, pancreas, kidney and lung. Overexpressed in PHA-stimulated T-cells.
• Relevance: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.
• Function: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Product Categories/Family for SEPSECS recombinant protein

Metabolism

References

Complete sequencing and characterization of 21,243 full-length human cDNAs.Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.Nat. Genet. 36:40-45(2004)
https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:30605
https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=253305
https://www.genome.jp/dbget-bin/www_bget?hsa:51091
https://string-db.org/network/9606.ENSP00000371535
https://www.omim.org/entry/613009613009613009

NCBI and Uniprot Product Information

• NCBI GI #: 7652498
• NCBI GeneID: 51091
• UniProt Accession #: Q9HD40; Q0D2P3; Q17RT1; Q9NXZ5; Q9UGM9; Q9Y353; A8K8W1
• Molecular Weight: 55,726 Da
• NCBI Official Full Name: soluble liver antigen/liver pancreas antigen
• NCBI Official Synonym Full Names: Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
• NCBI Official Symbol: SEPSECS
• NCBI Official Synonym Symbols: LP; SLA; PCH2D; SLA/LP
• NCBI Protein Information: O-phosphoseryl-tRNA(Sec) selenium transferase; O-phosphoseryl-tRNA(Sec) selenium transferase; SLA-p35; SLA/LP autoantigen; soluble liver antigen; liver-pancreas antigen; UGA suppressor tRNA-associated protein; tRNA(Ser/Sec)-associated antigenic protein; s
• UniProt Protein Name: O-phosphoseryl-tRNA(Sec) selenium transferase
• Protein Family: O-phosphoseryl-tRNA(Sec) selenium transferase
• UniProt Gene Name: SEPSECS
• UniProt Synonym Gene Names: TRNP48; LP; Sec synthase; SepSecS; SLA
• UniProt Entry Name: SPCS_HUMAN

NCBI Description

The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011]

Uniprot Description

SLA/LP: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl- tRNA(Sec) required for selenoprotein biosynthesis. Defects in SEPSECS are the cause of pontocerebellar hypoplasia type 2D (PCH2D). PCH2D is a disorder characterized by postnatal onset of progressive atrophy of the cerebrum and cerebellum, microcephaly, profound mental retardation, spasticity, and variable seizures. Belongs to the SepSecS family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.9.1.2; Transferase; RNA-binding

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; transferase activity, transferring selenium-containing groups; tRNA binding; pyridoxal phosphate binding

Biological Process: selenocysteine incorporation

Disease: Pontocerebellar Hypoplasia, Type 2d