Mitochondrial respiratory chain complexes assembly protein AFG3 (AFG3) Recombinant Protein | AFG3 recombinant protein

Recombinant Saccharomyces cerevisiae Mitochondrial respiratory chain complexes assembly protein AFG3 (AFG3)

• Gene Names: AFG3; YTA10
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Mitochondrial respiratory chain complexes assembly protein AFG3 (AFG3); Recombinant Saccharomyces cerevisiae Mitochondrial respiratory chain complexes assembly protein AFG3 (AFG3); Recombinant Mitochondrial respiratory chain complexes assembly protein AFG3 (AFG3); Mitochondrial respiratory chain complexes assembly protein AFG3 EC= 3.4.24.-; ATPase family gene 3 protein Tat-binding homolog 10; AFG3 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-761
• Sequence: MMMWQRYARGAPRSLTSLSFGKASRISTVKPVLRSRMPVHQRLQTLSGLATRNTIHRSTQIRSFHISWTRLNENRPNKEGEGKNNGNKDNNSNKEDGKDKRNEFGSLSEYFRSKEFANTMFLTIGFTIIFTLLTPSSNNSGDDSNRVLTFQDFKTKYLEKGLVSKIYVVNKFLVEAELVNTKQVVSFTIGSVDIFEEQMDQIQDLLNIPPRDRIPIKYIERSSPFTFLFPFLPTIILLGGLYFITRKINSSPPNANGGGGGGLGGMFNVGKSRAKLFNKETDIKISFKNVAGCDEAKQEIMEFVHFLKNPGKYTKLGAKIPRGAILSGPPGTGKTLLAKATAGEANVPFLSVSGSEFVEMFVGVGASRVRDLFTQARSMAPSIIFIDEIDAIGKERGKGGALGGANDEREATLNQLLVEMDGFTTSDQVVVLAGTNRPDVLDNALMRPGRFDRHIQIDSPDVNGRQQIYLVHLKRLNLDPLLTDDMNNLSGKLATLTPGFTGADIANACNEAALIAARHNDPYITIHHFEQAIERVIAGLEKKTRVLSKEEKRSVAYHEAGHAVCGWFLKYADPLLKVSIIPRGQGALGYAQYLPPDQYLISEEQFRHRMIMALGGRVSEELHFPSVTSGAHDDFKKVTQMANAMVTSLGMSPKIGYLSFDQNDGNFKVNKPFSNKTARTIDLEVKSIVDDAHRACTELLTKNLDKVDLVAKELLRKEAITREDMIRLLGPRPFKERNEAFEKYLDPKSNTEPPEAPAATN
• Sequence Length: 761

• Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 6320854
• NCBI GeneID: 856737
• NCBI Accession #: NP_010933.1
• NCBI GenBank Nucleotide #: NM_001178908.1
• UniProt Accession #: P39925; D3DLR5
• Molecular Weight: 84,544 Da
• NCBI Official Full Name: Afg3p
• NCBI Official Symbol: AFG3
• NCBI Official Synonym Symbols: YTA10
• NCBI Protein Information: Afg3p
• UniProt Protein Name: Mitochondrial respiratory chain complexes assembly protein AFG3
• Protein Family: Mitochondrial respiratory chain complexes assembly protein
• UniProt Gene Name: AFG3
• UniProt Synonym Gene Names: YTA10
• UniProt Entry Name: AFG3_YEAST

Uniprot Description

Function: Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides. Ref.5 Ref.6

Cofactor: Binds 1 zinc ion per subunit

Potential.

Subunit structure: Component of the 850 kDa m-AAA protease complex (YTA10-12) which consists of multiple copies of RCA1 AND AFG3. Ref.5

Subcellular location: Mitochondrion membrane; Multi-pass membrane protein Ref.5.

Miscellaneous: Present with 3870 molecules/cell in log phase SD medium.

Sequence similarities: In the N-terminal section; belongs to the AAA ATPase family.In the C-terminal section; belongs to the peptidase M41 family.