Probable diguanylate cyclase YegE (yegE) Recombinant Protein | yegE recombinant protein

Recombinant Escherichia coli Probable diguanylate cyclase YegE (yegE) , partial

• Gene Names: yegE; ECK2061; JW2052
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Probable diguanylate cyclase YegE (yegE); Recombinant Escherichia coli Probable diguanylate cyclase YegE (yegE); partial; yegE recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,105

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16130007
• NCBI GeneID: 946600
• NCBI Accession #: NP_416571.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P38097; P76391
• Molecular Weight: 123,887 Da
• NCBI Official Full Name: putative diguanylate cyclase
• NCBI Official Symbol: yegE
• NCBI Official Synonym Symbols: ECK2061; JW2052
• NCBI Protein Information: putative diguanylate cyclase
• UniProt Protein Name: Probable diguanylate cyclase DgcE
• Protein Family: Probable diguanylate cyclase
• UniProt Gene Name: dgcE
• UniProt Synonym Gene Names: DGCCurated

NCBI Description

A yegE null mutation partially suppresses the motility defect of a yhjH mutation; yegE partial suppression is best at 28C, full yhjH suppression requires yegE and yedQ mutations; a yeaJ null mutation is a better yhjH suppressor at 37C; full yhjH suppression requires yegE, yedQ and yeaJ null mutations when grown at 37C (Pesavento, 2008). [More information is available at EcoGene: EG12396]. YegE is a GGDEF/EAL protein that is involved in regulation of the switch from flagellar motility to sessile behavior and curli expression . [More information is available at EcoCyc: EG12396].

Uniprot Description

Catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules (). Involved in the control of the switch from cell motility to adhesion via regulation of cellular levels of c-di-GMP (Probable). Part of a signaling cascade that regulates curli biosynthesis. The cascade is composed of two c-di-GMP control modules, in which c-di-GMP controlled by the DgcE/PdeH pair (module I) regulates the activity of the DgcM/PdeR pair (module II), which in turn regulates activity of the transcription factor MlrA and expression of the master biofilm regulator csgD (PubMed:23708798).