Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MYCN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
MYCN Polyclonal Antibody | anti-MYCN antibody
MYCN Polyclonal Antibody
IHC 1:50 - 1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MYCN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MYCN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using MYCN antibody (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using MYCN antibody (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using MYCN antibody (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using MYCN antibody (40x lens).)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Uniprot Description
N-Myc: a proto-oncogenic transcription factor. Plays a role in cell proliferation, apoptosis and in the development of human tumors.
Protein type: Oncoprotein; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 2p24.3
Cellular Component: nucleus; chromatin
Molecular Function: protein dimerization activity; protein binding; DNA binding; transcription factor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; branching morphogenesis of a tube; positive regulation of mesenchymal cell proliferation; negative regulation of astrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; lung development; cartilage condensation; embryonic skeletal morphogenesis
Disease: Feingold Syndrome 1; Tracheoesophageal Fistula With Or Without Esophageal Atresia
Research Articles on MYCN
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Product Notes
The MYCN mycn (Catalog #AAA125752) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The MYCN Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MYCN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB 1:500 - 1:2000 IHC 1:50 - 1:200. Researchers should empirically determine the suitability of the MYCN mycn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYCN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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