Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SLC9A3R1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Rabbit NHERF1 Polyclonal Antibody | anti-SLC9A3R1 antibody
NHERF1 Rabbit pab Antibody
Gene Names
SLC9A3R1; EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity purification
Synonyms
NHERF1; Polyclonal Antibody; NHERF1 Rabbit pab Antibody; SLC9A3R1; NHERF1 ; NHERF; anti-SLC9A3R1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
358
Applicable Applications for anti-SLC9A3R1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Western Blot (WB): 1/2000-5000
Immunohistochemistry (IHC): 1/50
Immunocytochemistry (ICC): 1/50
Immunoprecipitation (IP): 1/20
Flow Cytometry (FC/FACS): 1/50-100
Immunohistochemistry (IHC): 1/50
Immunocytochemistry (ICC): 1/50
Immunoprecipitation (IP): 1/20
Flow Cytometry (FC/FACS): 1/50-100
Immunogen
A Fusion protein of human NHERF1
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SLC9A3R1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SLC9A3R1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Related Product Information for anti-SLC9A3R1 antibody
Rabbit polyclonal to Human NHERF1
Na+/H+ exchanger regulatory factor (NHERF1 or EBP-50) is one of several related PDZ domain-containing proteins (1). NHERF1 was first identified as a necessary cofactor for cyclic AMP-associated inhibition of Na+/ H+ exchanger isoform 3 (NHE3) (2). NHERF1 is a multifunctional adaptor protein that interacts with receptors and ion transporters via its PDZ domains, and with the ERM family of proteins, including merlin, via its carboxy-terminus (2,3). NHERF1 may play an important role in breast cancer. Estrogen has been found to induce NHERF1 in estrogen receptor-positive breast cancer cells (2,3). Furthermore, NHERF1 has been shown to bind to PDGFR, which is activated in breast carcinomas. NHERF1 has been found to promote the formation of a ternary complex containing PTEN, NHERF1, and PDGFR. Therefore, NHERF1 may function to recruit PTEN to PDGFR to inhibit the activation of PI3K/Akt signaling in normal cells; this mechanism may be disrupted in cancer (4). NHERF1 also binds to the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as an ion channel and has disease-causing mutations in cystic fibrosis (5). Other proposed functions of NHERF1 include testicular differentiation, endosomal recycling, membrane targeting, protein sorting, and trafficking (6).
Na+/H+ exchanger regulatory factor (NHERF1 or EBP-50) is one of several related PDZ domain-containing proteins (1). NHERF1 was first identified as a necessary cofactor for cyclic AMP-associated inhibition of Na+/ H+ exchanger isoform 3 (NHE3) (2). NHERF1 is a multifunctional adaptor protein that interacts with receptors and ion transporters via its PDZ domains, and with the ERM family of proteins, including merlin, via its carboxy-terminus (2,3). NHERF1 may play an important role in breast cancer. Estrogen has been found to induce NHERF1 in estrogen receptor-positive breast cancer cells (2,3). Furthermore, NHERF1 has been shown to bind to PDGFR, which is activated in breast carcinomas. NHERF1 has been found to promote the formation of a ternary complex containing PTEN, NHERF1, and PDGFR. Therefore, NHERF1 may function to recruit PTEN to PDGFR to inhibit the activation of PI3K/Akt signaling in normal cells; this mechanism may be disrupted in cancer (4). NHERF1 also binds to the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as an ion channel and has disease-causing mutations in cystic fibrosis (5). Other proposed functions of NHERF1 include testicular differentiation, endosomal recycling, membrane targeting, protein sorting, and trafficking (6).
References
1. Donowitz, M. et al. (2005) J Physiol 567, 3-11. 2. Voltz, J.W. et al. (2001) Oncogene 20, 6309-14. 3. Stemmer-Rachamimov, A.O. et al. (2001) Am J Pathol 158, 57-62. 4. Takahashi, Y. et al. (2006) EMBO J 25, 910-20. 5. Wheeler, D. et al. (2007) J Biol Chem 282, 25076-87. 6. Weinman, E.J. et al. (2000) Am J Physiol Renal Physiol 279, F393-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
358
NCBI Official Full Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
NCBI Official Synonym Full Names
solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
NCBI Official Symbol
SLC9A3R1
NCBI Official Synonym Symbols
EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
NCBI Protein Information
Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; regulatory cofactor of Na(+)/H(+) exchanger; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-binding phosphoprotein 50; solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1
UniProt Protein Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Protein Family
UniProt Gene Name
SLC9A3R1
UniProt Synonym Gene Names
NHERF; NHERF1; NHERF-1; EBP50
UniProt Entry Name
NHRF1_HUMAN
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Product Notes
The SLC9A3R1 slc9a3r1 (Catalog #AAA125205) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NHERF1 Rabbit pab Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NHERF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Western Blot (WB): 1/2000-5000 Immunohistochemistry (IHC): 1/50 Immunocytochemistry (ICC): 1/50 Immunoprecipitation (IP): 1/20 Flow Cytometry (FC/FACS): 1/50-100. Researchers should empirically determine the suitability of the SLC9A3R1 slc9a3r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NHERF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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