RNA polymerase sigma-32 factor (rpoH) Recombinant Protein | rpoH recombinant protein

Recombinant Escherichia coli RNA polymerase sigma-32 factor (rpoH)

Cat. Num. AAA1214958

• Gene Names: rpoH; ECK3445; fam; hin; htpR; JW3426
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: RNA polymerase sigma-32 factor (rpoH); Recombinant Escherichia coli RNA polymerase sigma-32 factor (rpoH); rpoH recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-284aa; Full Length
• Sequence: MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYHGDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARELGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWEEQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16131333
• NCBI GeneID: 947970
• NCBI Accession #: NP_417918.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0AGB3; P00580; Q2M7C4
• Molecular Weight: 39.9 kDa
• NCBI Official Full Name: RNA polymerase, sigma 32 (sigma H) factor
• NCBI Official Symbol: rpoH
• NCBI Official Synonym Symbols: ECK3445; fam; hin; htpR; JW3426
• NCBI Protein Information: RNA polymerase, sigma 32 (sigma H) factor
• UniProt Protein Name: RNA polymerase sigma factor RpoH
• Protein Family: RNA polymerase sigma factor
• UniProt Gene Name: rpoH

NCBI Description

RpoH is phosphorylated at Tyr-260, in HTH DNA-binding region 4.2, attenuating activity. de-phosphorylated by Etp. rpoH can be deleted, but null mutants do not grow above 20C (Zhou, 1988). [More information is available at EcoGene: EG10897]. Sigma 32 is the primary sigma factor controlling the heat shock response during log-phase growth. [More information is available at EcoCyc: EG10897].

Uniprot Description

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes. Intracellular concentration of free RpoH protein increases in response to heat shock, which causes association with RNA polymerase (RNAP) and initiation of transcription of heat shock genes, including numerous global transcriptional regulators and genes involved in maintaining membrane functionality and homeostasis. RpoH is then quickly degraded, leading to a decrease in the rate of synthesis of heat shock proteins and shut-off of the heat shock response.