Rabbit PPP5C Polyclonal Antibody | anti-PPP5C antibody

PPP5C Antibody

Cat. Num. AAA9611457

• Gene Names: PPP5C; PP5; PPT; PPP5
• Reactivity: Human, Mouse, Rat; Predicted: Bovine, Horse, Dog, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PPP5C; Polyclonal Antibody; PPP5C Antibody; FLJ36922; FLJ55954; OTTHUMP00000165834; PP-T; PP5; PPP5; PPP5_HUMAN; Ppp5c; PPT; Protein phosphatase 5; catalytic subunit; Protein phosphatase T; Serine/threonine protein phosphatase 5; Serine/threonine-protein phosphatase 5; anti-PPP5C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Bovine, Horse, Dog, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PPP5C Antibody detects endogenous levels of PPP5C.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 499

• Applicable Applications for anti-PPP5C antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human PPP5C.
• Tissue Specificity: Ubiquitous.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus; Plasma Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse testis tissue, using PPP5C Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-PPP5C antibody

Description: Serine/threonine-protein phosphatase that dephosphorylates a myriad of proteins involved in different signaling pathways including the kinases CSNK1E, ASK1/MAP3K5, PRKDC and RAF1, the nuclear receptors NR3C1, PPARG, ESR1 and ESR2, SMAD proteins and TAU/MAPT. Implicated in wide ranging cellular processes, including apoptosis, differentiation, DNA damage response, cell survival, regulation of ion channels or circadian rhythms, in response to steroid and thyroid hormones, calcium, fatty acids, TGF-beta as well as oxidative and genotoxic stresses. Participates in the control of DNA damage response mechanisms such as checkpoint activation and DNA damage repair through, for instance, the regulation ATM/ATR-signaling and dephosphorylation of PRKDC and TP53BP1. Inhibits ASK1/MAP3K5-mediated apoptosis induced by oxidative stress. Plays a positive role in adipogenesis, mainly through the dephosphorylation and activation of PPARG transactivation function. Also dephosphorylates and inhibits the anti-adipogenic effect of NR3C1. Regulates the circadian rhythms, through the dephosphorylation and activation of CSNK1E. May modulate TGF-beta signaling pathway by the regulation of SMAD3 phosphorylation and protein expression levels. Dephosphorylates and may play a role in the regulation of TAU/MAPT. Through their dephosphorylation, may play a role in the regulation of ions channels such as KCNH2.

Post Translational Modifications: Activated by at least two different proteolytic cleavages producing a 56 kDa and a 50 kDa form.

Subunit Structure: Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2 (PubMed:29127155). Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23 (PubMed:29127155). Part of a complex with HSP90/HSP90AA1 and steroid receptors (By similarity). Interacts (via TPR repeats) with HSP90AA1 (via TPR repeat-binding motif) or HSPA1A/HSPA1B; the interaction is direct and activates the phosphatase activity (PubMed:15383005, PubMed:15577939, PubMed:16531226). Dissociates from HSPA1A/HSPA1B and HSP90AA1 in response to arachidonic acid (PubMed:15383005). Interacts with CPNE1 (via VWFA domain) (By similarity). Interacts with CDC16, CDC27 (PubMed:9405394). Interacts with KLHDC10 (via the 6 Kelch repeats); inhibits the phosphatase activity on MAP3K5 (PubMed:23102700). Interacts with ATM and ATR; both interactions are induced by DNA damage and enhance ATM and ATR kinase activity (PubMed:14871926). Interacts with RAD17; reduced by DNA damage (PubMed:14871926). Interacts with nuclear receptors such as NR3C1/GCR and PPARG (activated by agonist); regulates their transactivation activities (By similarity). Interacts (via TPR repeats) with S100 proteins S100A1, S100A2, S100A6, S100B and S100P; the interactions are calcium-dependent, strongly activate PPP5C phosphatase activity and compete with HSP90AA1 and MAP3K5 interactions (PubMed:22399290). Interacts with SMAD2 and SMAD3 but not with SMAD1; decreases SMAD3 phosphorylation and protein levels (PubMed:22781750). Interacts (via TPR repeats) with CRY1 and CRY2; the interaction with CRY2 downregulates the phosphatase activity on CSNK1E (PubMed:16790549). Interacts (via TPR repeats) with the active form of RAC1, GNA12 or GNA13; these interactions activate the phosphatase activity and translocate PPP5C to the cell membrane (PubMed:19948726). Interacts with FLCN (PubMed:27353360).

Similarity: Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 5453958
• NCBI GeneID: 5536
• NCBI Accession #: NP_006238.1
• NCBI GenBank Nucleotide #: NP_006238.1
• UniProt Accession #: P53041
• Molecular Weight: Observed: 57 kDa; Predicted: 57 kDa
• NCBI Official Full Name: serine/threonine-protein phosphatase 5 isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 5 catalytic subunit
• NCBI Official Symbol: PPP5C
• NCBI Official Synonym Symbols: PP5; PPT; PPP5
• NCBI Protein Information: serine/threonine-protein phosphatase 5
• UniProt Protein Name: Serine/threonine-protein phosphatase 5
• UniProt Gene Name: PPP5C
• UniProt Synonym Gene Names: PPP5; PP5; PP-T; PPT
• UniProt Entry Name: PPP5_HUMAN

NCBI Description

This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

Uniprot Description

Function: May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.

Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.

Cofactor: Binds 1 iron ion per subunit

By similarity.Binds 1 manganese ion per subunit

By similarity.

Subunit structure: Interacts with CDC16 and CDC27. Ref.8

Subcellular location: Nucleus. Cytoplasm. Note: Predominantly nuclear. But also present in the cytoplasm.

Tissue specificity: Ubiquitous.

Sequence similarities: Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.Contains 3 TPR repeats.