Histone-lysine N-methyltransferase SUV39H1 (SUV39H1) Recombinant Protein | SUV39H1 recombinant protein

Recombinant Human Histone-lysine N-methyltransferase SUV39H1 (SUV39H1)

Cat. Num. AAA1164760

• Gene Names: SUV39H1; MG44; KMT1A; SUV39H; H3-K9-HMTase 1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Histone-lysine N-methyltransferase SUV39H1 (SUV39H1); Recombinant Human Histone-lysine N-methyltransferase SUV39H1 (SUV39H1); SUV39H1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-412, Full length protein
• Sequence: MAENLKGCSVCCKSSWNQLQDLCRLAKLSCPALGISKRNLYDFEVEYLCDYKKIREQEYYLVKWRGYPDSESTWEPRQNLKCVRILKQFHKDLERELLRRHHRSKTPRHLDPSLANYLVQKAKQRRALRRWEQELNAKRSHLGRITVENEVDLDGPPRAFVYINEYRVGEGITLNQVAVGCECQDCLWAPTGGCCPGASLHKFAYNDQGQVRLRAGLPIYECNSRCRCGYDCPNRVVQKGIRYDLCIFRTDDGRGWGVRTLEKIRKNSFVMEYVGEIITSEEAERRGQIYDRQGATYLFDLDYVEDVYTVDAAYYGNISHFVNHSCDPNLQVYNVFIDNLDERLPRIAFFATRTIRAGEELTFDYNMQVDPVDMESTRMDSNFGLAGLPGSPKKRVRIECKCGTESCRKYLF
• Sequence Length: 412

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for SUV39H1 recombinant protein

This gene is a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. This nuclear protein moves to the centromeres during mitosis and functions as a histone methyltransferase, methylating Lys-9 of histone H3. Overall, it plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression.

NCBI and Uniprot Product Information

• NCBI GI #: 531990830
• NCBI GeneID: 6839
• NCBI Accession #: NP_001269095.1
• NCBI GenBank Nucleotide #: NM_001282166.1
• UniProt Accession #: O43463; Q53G60; Q6FHK6; B2R6E8; B4DST0
• Molecular Weight: 49,148 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SUV39H1 isoform 1
• NCBI Official Synonym Full Names: suppressor of variegation 3-9 homolog 1
• NCBI Official Symbol: SUV39H1
• NCBI Official Synonym Symbols: MG44; KMT1A; SUV39H; H3-K9-HMTase 1
• NCBI Protein Information: histone-lysine N-methyltransferase SUV39H1
• UniProt Protein Name: Histone-lysine N-methyltransferase SUV39H1
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SUV39H1
• UniProt Synonym Gene Names: KMT1A; SUV39H; H3-K9-HMTase 1; Su(var)3-9 homolog 1

NCBI Description

This gene encodes an evolutionarily-conserved protein containing an N-terminal chromodomain and a C-terminal SET domain. The encoded protein is a histone methyltransferase that trimethylates lysine 9 of histone H3, which results in transcriptional gene silencing. Loss of function of this gene disrupts heterochromatin formation and may cause chromosome instability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.