HNRNP arginine N-methyltransferase (HMT1) Recombinant Protein | HMT1 recombinant protein

Recombinant Saccharomyces cerevisiae HNRNP arginine N-methyltransferase (HMT1)

Cat. Num. AAA1159328

• Gene Names: HMT1; HCP1; ODP1; RMT1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: HNRNP arginine N-methyltransferase (HMT1); Recombinant Saccharomyces cerevisiae HNRNP arginine N-methyltransferase (HMT1); HMT1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-348, full length protein
• Sequence: MSKTAVKDSATEKTKLSESEQHYFNSYDHYGIHEEMLQDTVRTLSYRNAIIQNKDLFKDKIVLDVGCGTGILSMFAAKHGAKHVIGVDMSSIIEMAKELVELNGFSDKITLLRGKLEDVHLPFPKVDIIISEWMGYFLLYESMMDTVLYARDHYLVEGGLIFPDKCSIHLAGLEDSQYKDEKLNYWQDVYGFDYSPFVPLVLHEPIVDTVERNNVNTTSDKLIEFDLNTVKISDLAFKSNFKLTAKRQDMINGIVTWFDIVFPAPKGKRPVEFSTGPHAPYTHWKQTIFYFPDDLDAETGDTIEGELVCSPNEKNNRDLNIKISYKFESNGIDGNSRSRKNEGSYLMH
• Sequence Length: 348

• Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 6319508
• NCBI GeneID: 852322
• NCBI Accession #: NP_009590.1
• NCBI GenBank Nucleotide #: NM_001178382.1
• UniProt Accession #: P38074; D6VQ34
• Molecular Weight: 39,786 Da
• NCBI Official Full Name: protein-arginine omega-N methyltransferase HMT1
• NCBI Official Symbol: HMT1
• NCBI Official Synonym Symbols: HCP1; ODP1; RMT1
• NCBI Protein Information: protein-arginine omega-N methyltransferase HMT1
• UniProt Protein Name: Protein arginine N-methyltransferase 1
• Protein Family: Heavy metal tolerance protein
• UniProt Gene Name: HMT1
• UniProt Synonym Gene Names: Type I PRMT

Uniprot Description

S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that catalyzes both the mono- and asymmetric (type I) dimethylation of the guanidino nitrogens of arginine residues in a variety of RNA-binding proteins such as heterogeneous nuclear ribonucleoproteins (hnRNPs) and small nuclear ribonucleoproteins (snRNPs) (PubMed:8668183, PubMed:8647869). Methylates NAB2, NPL3, HRP1 and YRA1, shuttling hnRNPs involved in mRNA processing and export, facilitating their export out of the nucleus (PubMed:8668183, PubMed:9499403, PubMed:10652296, PubMed:11779864, PubMed:15314027). Methylation of NPL3 weakens its interaction with THO2, a component of the TREX (transcription/export) complex important for transcriptional elongation and recruitment of mRNA export factors (PubMed:15314027, PubMed:20053728). Methylates the hnRNP HRB1, but does not influence its subcellular location (PubMed:9499403). Methylates the nucleolar proteins GAR1, NOP1 and NSR1 (PubMed:12756332). Methylates the snRNP SNP1 and modulates the cotranscriptional recruitment of splicing factors (PubMed:20823272). Dimethylates free histone H4 (HHF1/HHF2) at 'Arg-4' (H4R3me2a) and plays a role in preservation and establishment of silent chromatin domains (PubMed:12097318, PubMed:17158743). Mono- and dimethylates ribosomal protein S2 (RPS2) at 'Arg-11' (PubMed:20035717). Methylates the catalytic subunit of the SWI/SNF chromatin-remodeling complex SNF2 (PubMed:22997150).