Trans-activator protein BZLF1 Recombinant Protein | BZLF1 recombinant protein

Recombinant Epstein-Barr virus Trans-activator protein BZLF1 (BZLF1)

Cat. Num. AAA1146552

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Trans-activator protein BZLF1; Recombinant Epstein-Barr virus Trans-activator protein BZLF1 (BZLF1); Zebra; BZLF1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-245aa; Full Length
• Sequence: MMDPNSTSEDVKFTPDPYQVPFVQAFDQATRVYQDLGGPSQAPLPCVLWPVLPEPLPQGQLTAYHVSTAPTGSWFSAPQPAPENAYQAYAAPQLFPVSDITQNQQTNQAGGEAPQPGDNSTVQTAAAVVFACPGANQGQQLADIGVPQPAPVAAPARRTRKPQQPESLEECDSELEIKRYKNRVASRKCRAKFKQLLQHYREVAAAKSSENDRLRLLLKQMCPSLDVDSIIPRTPDVLHEDLLNF

• Production Note: Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for BZLF1 recombinant protein

Plays a key role in the switch from latent infection to lytic cycle producing new virions. Acts as a transcription factor, inducing early lytic cycle genes, and as a origin binding protein for genome replication. BZLF1 activates the promoter of another EBV gene (BSLF2+BMLF1).

References

DNA sequence and expression of the B95-8 Epstein-Barr virus genome.Baer R., Bankier A.T., Biggin M.D., Deininger P.L., Farrell P.J., Gibson T.J., Hatfull G., Hudson G.S., Satchwell S.C., Seguin C., Tuffnell P.S., Barrell B.G.Nature 310:207-211(1984) Epstein-Barr virus gene expression in P3HR1-superinfected Raji cells.Biggin M., Bodescot M., Perricaudet M., Farrel P.J. Virol. 61:3120-3132(1987) Epstein-Barr virus BZLF1 trans-activator specifically binds to a consensus AP-1 site and is related to c-fos.Farrel P.J., Rowe D.T., Rooney C.M., Kouzarides T.EMBO J. 8:127-132(1989) Structure and function of the Epstein-Barr virus BZLF1 protein.Packham G., Economou A., Rooney C.M., Rowe D.T., Farrel P.J.J. Virol. 64:2110-2116(1990) The BZLF1 protein of EBV has a coiled coil dimerisation domain without a heptad leucine repeat but with homology to the C/EBP leucine zipper.Kouzarides T., Packham G., Cook A., Farrell P.J.Oncogene 6:195-204(1991) A transcription factor with homology to the AP-1 family links RNA transcription and DNA replication in the lytic cycle of Epstein-Barr virus.Schepers A., Pich D., Hammerschmidt W.EMBO J. 12:3921-3929(1993) The PKC targeting protein RACK1 interacts with the Epstein-Barr virus activator protein BZLF1.Baumann M., Gires O., Kolch W., Mischak H., Zeidler R., Pich D., Hammerschmidt W.Eur. J. Biochem. 267:3891-3901(2000) Ubinuclein, a novel nuclear protein interacting with cellular and viral transcription factors.Aho S., Buisson M., Pajunen T., Ryoo Y.W., Giot J.-F., Gruffat H., Sergeant A., Uitto J.J. Cell Biol. 148:1165-1176(2000) Epstein-Barr virus BZLF1 gene, a switch from latency to lytic infection, is expressed as an immediate-early gene after primary infection of B lymphocytes.Wen W., Iwakiri D., Yamamoto K., Maruo S., Kanda T., Takada K.J. Virol. 81:1037-1042(2007) TORC2, a coactivator of cAMP-response element-binding protein, promotes Epstein-Barr virus reactivation from latency through interaction with viral BZLF1 protein.Murata T., Sato Y., Nakayama S., Kudoh A., Iwahori S., Isomura H., Tajima M., Hishiki T., Ohshima T., Hijikata M., Shimotohno K., Tsurumi T.J. Biol. Chem. 284:8033-8041(2009) Interaction of Epstein-Barr virus BZLF1 C-terminal tail structure and core zipper is required for DNA replication but not for promoter transactivation.McDonald C.M., Petosa C., Farrell P.J.J. Virol. 83:3397-3401(2009) Structural basis of lytic cycle activation by the Epstein-Barr virus ZEBRA protein.Petosa C., Morand P., Baudin F., Moulin M., Artero J.B., Muller C.W.Mol. Cell 21:565-572(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 82503229
• NCBI GeneID: 3783744
• NCBI Accession #: YP_401673.1
• NCBI GenBank Nucleotide #: NC_007605.1
• UniProt Accession #: P03206; Q69127; Q777E5; S03634
• Molecular Weight: 42.9 kDa
• NCBI Official Full Name: BZLF1
• NCBI Official Symbol: BZLF1
• NCBI Protein Information: BZLF1
• UniProt Protein Name: Trans-activator protein BZLF1
• Protein Family: Trans-activator protein
• UniProt Gene Name: BZLF1
• UniProt Synonym Gene Names: EB1
• UniProt Entry Name: BZLF1_EBVB9

Uniprot Description

Plays a key role in the switch from latent infection to lytic cycle producing new virions. Acts as a transcription factor, inducing early lytic cycle genes, and as a origin binding protein for genome replication. BZLF1 activates the promoter of another EBV gene (BSLF2+BMLF1).