DNA repair protein XRCC3 Recombinant Protein | XRCC3 recombinant protein

Recombinant Human DNA repair protein XRCC3

Cat. Num. AAA1122399

• Gene Names: XRCC3; CMM6
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA repair protein XRCC3; Recombinant Human DNA repair protein XRCC3; X-ray repair cross-complementing protein 3; XRCC3 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-316aa; Full Length
• Sequence: MDLDLLDLNPRIIAAIKKAKLKSVKEVLHFSGPDLKRLTNLSSPEVWHLLRTASLHLRGSSILTALQLHQQKERFPTQHQRLSLGCPVLDALLRGGLPLDGITELAGRSSAGKTQLALQLCLAVQFPRQHGGLEAGAVYICTEDAFPHKRLQQLMAQQPRLRTDVPGELLQKLRFGSQIFIEHVADVDTLLECVNKKVPVLLSRGMARLVVIDSVAAPFRCEFDSQASAPRARHLQSLGATLRELSSAFQSPVLCINQVTEAMEEQGAAHGPLGFWDERVSPALGITWANQLLVRLLADRLREEEAALGCPARTLR
• Sequence Length: 346

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for XRCC3 recombinant protein

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD21 paralog protein complex CX3 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and ses to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.

Product Categories/Family for XRCC3 recombinant protein

Epigenetics and Nuclear Signaling

References

XRCC2 and XRCC3, new human Rad51-family members, promote chromosome stability and protect against DNA cross-links and other damages.Liu N., Lamerdin J.E., Tebbs R.S., Schild D., Tucker J.D., Shen M.R., Brookman K.W., Siciliano M.J., Walter C.A., Fan W., Narayana L.S., Zhou Z.-Q., Adamson A.W., Sorensen K.J., Chen D.J., Jones N.J., Thompson L.H.Mol. Cell 1:783-793(1998) NIEHS SNPs program Identification and purification of two distinct complexes containing the five RAD51 paralogs.Masson J.Y., Tarsounas M.C., Stasiak A.Z., Stasiak A., Shah R., McIlwraith M.J., Benson F.E., West S.C.Genes Dev. 15:3296-3307(2001) Variants in DNA double-strand break repair genes and breast cancer susceptibility.Kuschel B., Auranen A., McBride S., Novik K.L., Antoniou A., Lipscombe J.M., Day N.E., Easton D.F., Ponder B.A., Pharoah P.D., Dunning A.Hum. Mol. Genet. 11:1399-1407(2002) RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51.Miller K.A., Yoshikawa D.M., McConnell I.R., Clark R., Schild D., Albala J.S.J. Biol. Chem. 277:8406-8411(2002) Involvement of Rad51C in two distinct protein complexes of Rad51 paralogs in human cells.Liu N., Schild D., Thelen M.P., Thompson L.H.Nucleic Acids Res. 30:1009-1015(2002) Domain mapping of the Rad51 paralog protein complexes.Miller K.A., Sawicka D., Barsky D., Albala J.S.Nucleic Acids Res. 32:169-178(2004) RAD51C is required for Holliday junction processing in mammalian cells.Liu Y., Masson J.Y., Shah R., O'Regan P., West S.C.Science 303:243-246(2004) Cellular localization of human Rad51C and regulation of ubiquitin-mediated proteolysis of Rad51.Bennett B.T., Knight K.L.J. Cell. Biochem. 96:1095-1109(2005) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.Science 316:1160-1166(2007) Discovery of a novel function for human Rad51 maintenance of the mitochondrial genome.Sage J.M., Gildemeister O.S., Knight K.L.J. Biol. Chem. 285:18984-18990(2010) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) hSWS1.SWSAP1 is an evolutionarily conserved complex required for efficient homologous recombination repair.Liu T., Wan L., Wu Y., Chen J., Huang J.J. Biol. Chem. 286:41758-41766(2011) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) The RAD51 paralogs ensure cellular protection against mitotic defects and aneuploidy.Rodrigue A., Coulombe Y., Jacquet K., Gagne J.P., Roques C., Gobeil S., Poirier G., Masson J.Y.J. Cell Sci. 126:348-359(2013) Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway.Chun J., Buechelmaier E.S., Powell S.N.Mol. Cell. Biol. 33:387-395(2013) Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair.Park J.Y., Singh T.R., Nassar N., Zhang F., Freund M., Hanenberg H., Meetei A.R., Andreassen P.R.Oncogene 33:4803-4812(2014) Ring-shaped Rad51 paralog protein complexes bind Holliday junctions and replication forks as visualized by electron microscopy.Compton S.A., Ozgur S., Griffith J.D.J. Biol. Chem. 285:13349-13356(2010) A variant within the DNA repair gene XRCC3 is associated with the development of melanoma skin cancer.Winsey S.L., Haldar N.A., Marsh H.P., Bunce M., Marshall S.E., Harris A.L., Wojnarowska F., Welsh K.I.Cancer Res. 60:5612-5616(2000)

NCBI and Uniprot Product Information

• NCBI GI #: 153946427
• NCBI GeneID: 7517
• NCBI Accession #: NP_001093588.1
• NCBI GenBank Nucleotide #: NM_001100118.1
• UniProt Accession #: O43542; O43568; Q9BU18
• Molecular Weight: 50.8 kDa
• NCBI Official Full Name: DNA repair protein XRCC3
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 3
• NCBI Official Symbol: XRCC3
• NCBI Official Synonym Symbols: CMM6
• NCBI Protein Information: DNA repair protein XRCC3
• UniProt Protein Name: DNA repair protein XRCC3
• Protein Family: DNA repair protein
• UniProt Gene Name: XRCC3
• UniProt Entry Name: XRCC3_HUMAN

NCBI Description

This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

XRCC3: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C. Defects in XRCC3 are the cause of susceptibility to breast cancer (BC). BC is a common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Defects in XRCC3 are the cause of susceptibility to cutaneous malignant melanoma type 6 (CMM6). CMM6 is a malignant neoplasm of melanocytes, arising de novo or from a pre- existing benign nevus, which occurs most often in the skin but also may involve other sites. Belongs to the RecA family. RAD51 subfamily.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 14q32.3

Cellular Component: cytoplasm; mitochondrion; nuclear chromosome, telomeric region; nucleoplasm; nucleus; perinuclear region of cytoplasm; replication fork

Molecular Function: ATP binding; crossover junction endodeoxyribonuclease activity; DNA-dependent ATPase activity; double-stranded DNA binding; four-way junction DNA binding; protein binding; recombinase activity; single-stranded DNA binding

Biological Process: DNA recombination; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via synthesis-dependent strand annealing; meiotic DNA recombinase assembly; meiotic recombination; response to DNA damage stimulus; response to ionizing radiation; response to organic substance; strand invasion; telomere maintenance via recombination

Disease: Breast Cancer; Melanoma, Cutaneous Malignant, Susceptibility To, 6