Capsid Recombinant Protein | C,HBcAg recombinant protein

Recombinant Hepatitis B virus genotype C subtype ayw (isolate China/Tibet127/2002) (HBV-C) Capsid protein

Cat. Num. AAA1093382

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Capsid; Recombinant Hepatitis B virus genotype C subtype ayw (isolate China/Tibet127/2002) (HBV-C) Capsid protein; Core antigen; Core protein; HBcAgp21.5; C; HBcAg recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-183aa; Full Length Protein
• Sequence: MDIDPYKEFGASVEVLSFLPSDFFPSNRDLLDTASALDREALESPEHCSPHHTALRQAILCWGELMNLATWVGSNLEDPASRELVVSYVNVNMGLKIRQLLWFHISCLTFGRETVLEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVVRRRGRSPRRRTPSPRRRRSQSPRRRRSQSRESQC

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-Page

Related Product Information for C,HBcAg recombinant protein

Self assbles to form an icosahedral capsid. Most capsid appear to be large particles with a icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsid are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. Only capsids that contain the mature viral genome can release the viral DNA and capsid protein into the nucleoplasm. Immature capsids get stucked in the basket. Capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus, providing more genome for transcription, or bud through the endoplasmic reticulum to provide new virions. Encapsidates hepatitis delta genome.

References

The dominant hepatitis B virus genotype identified in Tibet is a C/D hybrid.Cui C., Shi J., Hui L., Xi H., Zhuoma X., Quni X., Tsedan X., Hu G.J. Gen. Virol. 83:2773-2777(2002)

NCBI and Uniprot Product Information

• NCBI GI #: 170652933
• UniProt Accession #: P0C6H7
• Molecular Weight: 23 kDa
• NCBI Official Full Name: Capsid protein
• UniProt Protein Name: Capsid protein
• Protein Family: Capsid protein
• UniProt Gene Name: C
• UniProt Entry Name: CAPSD_HBVC7

Uniprot Description

Self assembles to form an icosahedral capsid. Most capsid appear to be large particles with a icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsid are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. Only capsids that contain the mature viral genome can release the viral DNA and capsid protein into the nucleoplasm. Immature capsids get stucked in the basket. Capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus, providing more genome for transcription, or bud through the endoplasmic reticulum to provide new virions ().