DNA-directed RNA polymerase I subunit RPA1 (POLR1A) Recombinant Protein | POLR1A recombinant protein

Recombinant Human DNA-directed RNA polymerase I subunit RPA1 (POLR1A) , partial

Cat. Num. AAA1056774

• Gene Names: POLR1A; A190; RPA1; RPO14; AFDCIN; RPA194; RPO1-4
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA-directed RNA polymerase I subunit RPA1 (POLR1A); Recombinant Human DNA-directed RNA polymerase I subunit RPA1 (POLR1A); partial; POLR1A recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1198-1497, Partial
• Sequence: LKWQRSLCEPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKALKRVKSLKKQLTRVCLGEVLQKIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQEKCLRPEDILRFMETRFFKLLMESIKKKNNKASAFRNVNTRRATQRDLDNAGELGRSRGEQEGDEEEEGHIVDAEAEEGDADASDAKRKEKQEEEVDYESEEEEEREGEENDDEDMQEERNPHREGARKTQEQDEEVGLGTEEDPSLPALLTQPRKPTHSQEPQGPEA
• Sequence Length: 1497

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 103471997
• NCBI GeneID: 25885
• NCBI Accession #: NP_056240.2
• NCBI GenBank Nucleotide #: NM_015425.5
• UniProt Accession #: O95602; Q0VG05; Q9UEH0; Q9UFT9; B7Z7T0; D6W5M0
• Molecular Weight: 194,811 Da
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA1
• NCBI Official Synonym Full Names: RNA polymerase I subunit A
• NCBI Official Symbol: POLR1A
• NCBI Official Synonym Symbols: A190; RPA1; RPO14; AFDCIN; RPA194; RPO1-4
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA1
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA1
• UniProt Gene Name: POLR1A
• UniProt Synonym Gene Names: RNA polymerase I subunit A1; RPA194

NCBI Description

The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016]

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition ().