Chicken FK506 Binding Protein 12 Rapamycin Associated Protein ELISA Kit | FRAP elisa kit

Chicken FK506 Binding Protein 12 Rapamycin Associated Protein ELISA Kit

Cat. Num. AAA104725

• Gene Names: MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
• Reactivity: Chicken
• Synonyms: FK506 Binding Protein 12 Rapamycin Associated Protein; Chicken FK506 Binding Protein 12 Rapamycin Associated Protein ELISA Kit; Serine/threonine-protein kinase mTOR; mTOR; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12; FRAP elisa kit

• Reactivity: Chicken
• Sequence Length: 2583

• Assay Type: Quantitative Sandwich
• Detection Range: 6.25ng/ml-200ng/ml
• Sensitivity: 1.0ng/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for FRAP elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used for determine the level of FRAP (hereafter termed "analyte") in undiluted original Chicken serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 68533045
• NCBI GeneID: 2475
• UniProt Accession #: P42345; Q4LE76; Q5TER1; Q6LE87; Q96QG3; Q9Y4I3
• Molecular Weight: 288,892 Da
• NCBI Official Full Name: FRAP1 variant protein, partial
• NCBI Official Synonym Full Names: mechanistic target of rapamycin (serine/threonine kinase)
• NCBI Official Symbol: MTOR
• NCBI Official Synonym Symbols: FRAP; FRAP1; FRAP2; RAFT1; RAPT1
• NCBI Protein Information: serine/threonine-protein kinase mTOR; rapamycin target protein 1; mammalian target of rapamycin; rapamycin and FKBP12 target 1; FKBP-rapamycin associated protein; rapamycin associated protein FRAP2; FKBP12-rapamycin complex-associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1
• UniProt Protein Name: Serine/threonine-protein kinase mTOR
• UniProt Gene Name: MTOR
• UniProt Synonym Gene Names: FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR
• UniProt Entry Name: MTOR_HUMAN

NCBI Description

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

mTOR: an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779).

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; Autophagy; ATYPICAL group; PIKK family; FRAP subfamily

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: PML body; endoplasmic reticulum membrane; lysosome; lysosomal membrane; dendrite; endomembrane system; cytosol; nucleoplasm; Golgi membrane; mitochondrial outer membrane; membrane; cell soma; cytoplasm; TORC2 complex; nucleus; phosphoinositide 3-kinase complex

Molecular Function: protein dimerization activity; protein domain specific binding; protein serine/threonine kinase activity; protein binding; ribosome binding; phosphoprotein binding; kinase activity; drug binding; ATP binding

Biological Process: negative regulation of autophagy; TOR signaling pathway; regulation of myelination; positive regulation of translation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; negative regulation of cell size; signal transduction; protein amino acid phosphorylation; germ cell development; negative regulation of macroautophagy; cellular response to nutrient levels; positive regulation of stress fiber formation; regulation of carbohydrate utilization; response to stress; protein catabolic process; cell growth; regulation of response to food; response to nutrient; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; peptidyl-threonine phosphorylation; positive regulation of lipid biosynthetic process; negative regulation of NFAT protein import into nucleus; response to amino acid stimulus; double-strand break repair via homologous recombination; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; insulin receptor signaling pathway; ruffle organization and biogenesis; regulation of fatty acid beta-oxidation; positive regulation of transcription from RNA polymerase III promoter; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; regulation of protein kinase activity; vascular endothelial growth factor receptor signaling pathway; phosphorylation; growth