CHN1 recombinant protein

CHN1 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8121785

• Purity: >90% as determined by SDS-PAGE
• Synonyms: CHN1; CHN1 Protein; Human; Recombinant (His & GST Tag); Human CHN1/Chimerin 1 Protein (His & GST Tag); ARHGAP2 Protein; CHN Protein; DURS2 Protein; NC Protein; RHOGAP2 Protein; chimerin 1; CHN1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala2-Phe459

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human CHN1 (NP_001813.1)(Ala2-Phe459) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CHN1 recombinant protein

Background: CHN1, also known as chimerin 1, is a TPase-activating protein for ras-related p21-rac and a phorbol ester receptor. It is predominantly expressed in neurons, and plays an important role in neuronal signal-transduction mechanisms. CHN1 is involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance. The CHN1 gene provides instructions for making two very similar proteins called alpha1-chimaerin and alpha2-chimaerin. These proteins play an important role in the early development of the nervous system. In particular, they help regulate complex chemical signaling pathways during the formation and development of nerve cells (neurons). These proteins help guide the growth of axons and dendrites, which are specialized extensions of neurons that transmit and receive nerve impulses throughout the nervous system.

References

Miyake N. et al, 2010, Am J Med Genet A. 152 (1): 215-7. Miyake N. et al., 2011, Invest Ophthalmol Vis Sci. 52 (9): 6321-8. Volk AE. et al., 2010, Graefes Arch Clin Exp Ophthalmol. 248 (9): 1351-7.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant human CHN1/GST chimera consists of 695 amino acids and has a calculated molecular mass of 80.9kDa. The recombinant protein migrates as an approximately 65kDa band in SDS-PAGE under reducing conditions.
• Protein Family: N-chimaerin