Human Receptor Interacting Serine Threonine Kinase 2 ELISA Kit | RIPK2 elisa kit

Human Receptor Interacting Serine Threonine Kinase 2 ELISA Kit

Cat. Num. AAA10017

• Gene Names: RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
• Reactivity: Human
• Synonyms: Receptor Interacting Serine Threonine Kinase 2; Human Receptor Interacting Serine Threonine Kinase 2 ELISA Kit; RIPK2 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human RIPK2 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml - 2000 pg/ml.
• Sensitivity: 10 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated RIPK2 concentrations in Human serum, plasma and other body fluids. Using Purified Human RIPK2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RIPK2 and RIPK2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RIPK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for RIPK2 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated RIPK2 concentrations in Human serum, plasma and other body fluids. Using Purified Human RIPK2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RIPK2 and RIPK2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RIPK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506537
• NCBI GeneID: 8767
• NCBI Accession #: NP_003812.1
• NCBI GenBank Nucleotide #: NM_003821.5
• UniProt Accession #: O43353; Q6UWF0
• Molecular Weight: 61,195 Da
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 2
• NCBI Official Synonym Full Names: receptor-interacting serine-threonine kinase 2
• NCBI Official Symbol: RIPK2
• NCBI Official Synonym Symbols: CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 2; RIP-2; CARD-carrying kinase; growth-inhibiting gene 30; tyrosine-protein kinase RIPK2; receptor-interacting protein 2; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 2
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: RIPK2
• UniProt Synonym Gene Names: CARDIAK; RICK; RIP2; CARD-containing IL-1 beta ICE-kinase; RIP-2
• UniProt Entry Name: RIPK2_HUMAN

NCBI Description

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

RIPK2: a tyrosine kinase-like kinase of the RIPK family. Activates pro-caspase-1 and pro-caspase-8. Potentiates casp-8-mediated apoptosis. May activate NF-kappaB.

Protein type: EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, TKL; EC 2.7.11.1; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytoskeleton; protein complex; cytoplasm; cytosol; vesicle

Molecular Function: protein serine/threonine kinase activity; LIM domain binding; signal transducer activity; protein binding; protein homodimerization activity; CARD domain binding; non-membrane spanning protein tyrosine kinase activity; ATP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; activation of MAPK activity; positive regulation of interleukin-12 production; positive regulation of apoptosis; apoptosis; stress-activated MAPK cascade; positive regulation of JNK cascade; toll-like receptor 3 signaling pathway; signal transduction; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; T cell proliferation; response to exogenous dsRNA; positive regulation of immature T cell proliferation; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon-beta production; JNK cascade; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; adaptive immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of peptidyl-serine phosphorylation; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein ubiquitination; toll-like receptor signaling pathway; positive regulation of interleukin-2 production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of alpha-beta T cell proliferation; toll-like receptor 9 signaling pathway; positive regulation of cytokine and chemokine mediated signaling pathway; positive regulation of T-helper 1 cell differentiation; negative regulation of apoptosis