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Flow Cytometry (FC/FACS) (Separation of Jurkat cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD90 (5E10) APC-Cy7 antibody (4 ul reagent per milion cells in 100 ul of cell suspension).)

Mouse CD90 Monoclonal Antibody | anti-CD90 antibody

Anti-Hu CD90 APC-Cyanine7

Gene Names
THY1; CD90; CDw90
Reactivity
Pig, Horse, Human, Non-human primates
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with tandem dye APC-Cyanine7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD90; Monoclonal Antibody; Anti-Hu CD90 APC-Cyanine7; Thy-1 cell surface antigen; Thy-1; anti-CD90 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Pig, Horse, Human, Non-human primates
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
5E10
Specificity
The mouse monoclonal antibody 5E10 recognizes CD90/Thy-1, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells and neurons.
Purity/Purification
The purified antibody is conjugated with tandem dye APC-Cyanine7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Sequence Length
161
Applicable Applications for anti-CD90 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Immunogen
HEL erythroleukemia cells
Negative Species
Dog
Conjugation
APC-Cyanine7
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Separation of Jurkat cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD90 (5E10) APC-Cy7 antibody (4 ul reagent per milion cells in 100 ul of cell suspension).)

Flow Cytometry (FC/FACS) (Separation of Jurkat cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD90 (5E10) APC-Cy7 antibody (4 ul reagent per milion cells in 100 ul of cell suspension).)
Related Product Information for anti-CD90 antibody
CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thy-1 membrane glycoprotein isoform 1 preproprotein
NCBI Official Synonym Full Names
Thy-1 cell surface antigen
NCBI Official Symbol
THY1
NCBI Official Synonym Symbols
CD90; CDw90
NCBI Protein Information
thy-1 membrane glycoprotein
UniProt Protein Name
Thy-1 membrane glycoprotein
UniProt Gene Name
THY1
UniProt Entry Name
THY1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: anchored to external side of plasma membrane; focal adhesion; growth cone; endoplasmic reticulum; integral to plasma membrane; dendrite; apical plasma membrane; plasma membrane; cytosol; lipid raft; external side of plasma membrane

Molecular Function: integrin binding; protein binding; protein kinase binding

Biological Process: focal adhesion formation; cell-cell adhesion; retinal cone cell development; negative regulation of T cell receptor signaling pathway; negative regulation of protein kinase activity; negative regulation of axonogenesis; cytoskeleton organization and biogenesis; angiogenesis; positive regulation of T cell activation; positive regulation of release of sequestered calcium ion into cytosol; T cell receptor signaling pathway; negative regulation of cell migration; positive regulation of GTPase activity

Research Articles on CD90

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Product Notes

The CD90 thy1 (Catalog #AAA1800256) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD90 APC-Cyanine7 reacts with Pig, Horse, Human, Non-human primates and may cross-react with other species as described in the data sheet. AAA Biotech's CD90 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD90 thy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD90, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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