Rat 5-Nucleotidase ELISA Kit | 5-NT elisa kit

Rat 5-Nucleotidase (5-NT) ELISA Kit

Cat. Num. AAA281234

• Gene Names: Nt5e; Nt5; CD73
• Reactivity: Rat
• Synonyms: 5-Nucleotidase; Rat 5-Nucleotidase (5-NT) ELISA Kit; 5-NT elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat 5-NT. No significant cross-reactivity or interference between Rat 5-NT and analogues was observed.
• Sequence Length: 576

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for 5-NT elisa kit

5'-nucleotidase (EC 3.1.3.5) is an enzyme which catalyzes the phosphorylytic cleavage of 5'nucleotides. Although originally found in snake venom, the activity of 5'nucleotidase has been described for bacteria and plant cells, and is widely distributed in vertebrate tissue. In mammalian cells the enzyme is predominantly located in the plasma membrane and its primary role is in the conversion of extracellular nucleotides (e.g. 5'-AMP), which are generally impermeable, to the corresponding nucleoside (e.g. adenosine) which can readily enter most cells. Consequently, the enzyme plays a key role in the metabolism of nucleotides.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate 5-NT in samples. An antibody specific for 5-NT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any5-NT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5-NT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5-NT bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 148747417
• NCBI GeneID: 58813
• NCBI Accession #: NP_067587.2
• NCBI GenBank Nucleotide #: NM_021576.2
• Molecular Weight: 63,969 Da
• NCBI Official Full Name: 5'-nucleotidase
• NCBI Official Synonym Full Names: 5' nucleotidase, ecto
• NCBI Official Symbol: Nt5e
• NCBI Official Synonym Symbols: Nt5; CD73
• NCBI Protein Information: 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: Nt5e
• UniProt Synonym Gene Names: Nt5; Nte; 5'-NT
• UniProt Entry Name: 5NTD_RAT

NCBI Description

may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Membrane protein, GPI anchor; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Membrane protein, integral

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane; integral to membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; ferrous iron binding; nucleotide binding

Biological Process: dephosphorylation; negative regulation of inflammatory response; adenosine biosynthetic process; brain development; purine nucleotide biosynthetic process; positive regulation of lipid biosynthetic process; adenosine metabolic process; response to aluminum ion; AMP catabolic process