Rat Arachidonate 5-Lipoxygenase ELISA Kit | 5-LO elisa kit

Rat Arachidonate 5-Lipoxygenase ELISA Kit

Cat. Num. AAA764689

• Gene Names: Alox5; 5-LO
• Reactivity: Rat
• Synonyms: Arachidonate 5-Lipoxygenase; Rat Arachidonate 5-Lipoxygenase ELISA Kit; 5-LO/Alox5/5-LO/5LPG/LOG5/LOX5A/5-lipoxygenase/5-LOX/arachidonic 5-lipoxygenase alpha-10 isoform/arachidonic 5-lipoxygenase delta-10-13 isoform/arachidonic 5-lipoxygenase delta-13 isoform/arachidonic 5-lipoxygenase delta-p10 isoform/arachidonic acid 5-lipoxygenase/leukotriene A4 synthase/LOG5; 5-LO elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of 2, 5-OAS. No significant cross-reactivity or interference between 2, 5-OAS and analogues was observed.
• Sequence Length: 673

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/ml
• Sensitivity: <0.469ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 2, 5-OAS were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 2, 5-OAS were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for 5-LO elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-2, 5-OAS antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-2, 5-OAS antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 2, 5-OAS amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of 2, 5-OAS can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 528078462
• NCBI GeneID: 101839970
• NCBI Accession #: NP_001268516.1
• NCBI GenBank Nucleotide #: NM_001281587.1
• Molecular Weight: 77,873 Da
• NCBI Official Full Name: arachidonate 5-lipoxygenase
• NCBI Official Symbol: Alox5
• NCBI Official Synonym Symbols: 5-LO
• NCBI Protein Information: arachidonate 5-lipoxygenase
• UniProt Protein Name: Arachidonate 5-lipoxygenase
• Protein Family: Arachidonate 5-lipoxygenase
• UniProt Gene Name: ALOX5
• UniProt Synonym Gene Names: 5-LO; 5-lipoxygenase
• UniProt Entry Name: LOX5_MESAU

Uniprot Description

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.