Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only)

Bovine 5-lipoxygenase ELISA Kit | 5-LO elisa kit

Bovine 5-lipoxygenase ELISA Kit

Gene Names
ALOX5; 5-LO; 5LPG; LOG5; 5-LOX
Reactivity
Bovine
Synonyms
5-lipoxygenase; Bovine 5-lipoxygenase ELISA Kit; 5-LO elisa kit
Ordering
For Research Use Only!
Reactivity
Bovine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for 5-LO elisa kit
Intended Uses: This 5-LO/LOX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine 5-LO/LOX. This ELISA kit for research use only!

Principle of the Assay: 5-LO/LOX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-5-LO/LOX antibody and an 5-LO/LOX-HRP conjugate. The assay sample and buffer are incubated together with 5-LO/LOX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 5-LO/LOX concentration since 5-LO/LOX from samples and 5-LO/LOX-HRP conjugate compete for the anti-5-LO/LOX antibody binding site. Since the number of sites is limited, as more sites are occupied by 5-LO/LOX from the sample, fewer sites are left to bind 5-LO/LOX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 5-LO/LOX concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
240
Molecular Weight
56,291 Da
NCBI Official Full Name
5-lipoxygenase, partial
NCBI Official Synonym Full Names
arachidonate 5-lipoxygenase
NCBI Official Symbol
ALOX5
NCBI Official Synonym Symbols
5-LO; 5LPG; LOG5; 5-LOX
NCBI Protein Information
arachidonate 5-lipoxygenase; LOX-5; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform
UniProt Protein Name
Arachidonate 5-lipoxygenase
UniProt Gene Name
ALOX5
UniProt Synonym Gene Names
LOG5; 5-LO; 5-lipoxygenase
UniProt Entry Name
LOX5_HUMAN

NCBI Description

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.

Protein type: Oxidoreductase; Nuclear envelope; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; EC 1.13.11.34

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol

Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity

Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Asthma, Susceptibility To

Research Articles on 5-LO

Similar Products

Product Notes

The Bovine 5-LO alox5 (Catalog #AAA740167) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA740167 ELISA Kit recognizes Bovine 5-LO. It is sometimes possible for the material contained within the vial of "5-lipoxygenase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.