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Sequence Information

Tumor Necrosis Factor Alpha (TNFa) Protein | TNFa protein

Eukaryotic Tumor Necrosis Factor Alpha (TNFa)

Gene Names
TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Tumor Necrosis Factor Alpha (TNFa); Eukaryotic Tumor Necrosis Factor Alpha (TNFa); DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2; TNFa protein
Ordering
For Research Use Only!
Host
293F cell
Purity/Purification
> 90%
Form/Format
Freeze-dried powder
Sequence Length
233
Applicable Applications for TNFa protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Fragment
Gly57~Leu235
Tag
N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
Source Note
Eukaryotic expression
Organism Species
Mouse
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
5
Subcellular Location
Membrane, Secreted
Usage
Reconstitute in PBS or others.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

Sequence Information

SDS-Page

SDS-Page

Testing Data

Testing Data
Product Categories/Family for TNFa protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21.4kDa; 24kDa
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
NCBI Protein Information
tumor necrosis factor
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2

NCBI Description

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Uniprot Description

TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Apoptosis; Cytokine; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: cell surface; external side of plasma membrane; extracellular region; extracellular space; integral to plasma membrane; lipid raft; phagocytic cup; plasma membrane; recycling endosome

Molecular Function: cytokine activity; identical protein binding; protease binding; protein binding; tumor necrosis factor receptor binding

Biological Process: activation of MAPK activity; activation of MAPKKK activity; activation of NF-kappaB transcription factor; caspase activation; chronic inflammatory response to antigenic stimulus; cortical actin cytoskeleton organization and biogenesis; embryonic gut development; I-kappaB kinase/NF-kappaB cascade; induction of apoptosis via death domain receptors; inflammatory response; leukocyte tethering or rolling; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; negative regulation of cytokine secretion during immune response; negative regulation of interleukin-6 production; negative regulation of lipid catabolic process; negative regulation of protein complex disassembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of cell adhesion; positive regulation of chemokine biosynthetic process; positive regulation of chemokine production; positive regulation of cytokine production; positive regulation of cytokine secretion; positive regulation of fever; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of JNK activity; positive regulation of MAP kinase activity; positive regulation of membrane protein ectodomain proteolysis; positive regulation of NF-kappaB import into nucleus; positive regulation of NFAT protein import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of phagocytosis; positive regulation of programmed cell death; positive regulation of protein amino acid phosphorylation; positive regulation of protein complex assembly; positive regulation of protein complex disassembly; positive regulation of protein kinase activity; positive regulation of protein transport; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein import into nucleus, translocation; protein kinase B signaling cascade; receptor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of insulin secretion; response to glucocorticoid stimulus; response to salt stress; response to virus; sequestering of triacylglycerol; tumor necrosis factor-mediated signaling pathway

Disease: Asthma, Susceptibility To; Malaria, Susceptibility To; Migraine With Or Without Aura, Susceptibility To, 1

Research Articles on TNFa

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Product Notes

The TNFa tnf (Catalog #AAA2086697) is a Protein produced from 293F cell and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tumor Necrosis Factor Alpha (TNFa) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the TNFa tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Alpha (TNFa), Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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