Erythropoietin (EPO) Protein | EPO protein

Eukaryotic Erythropoietin (EPO)

Cat. Num. AAA2086655

• Gene Names: EPO; EP; MVCD2
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Erythropoietin (EPO); Eukaryotic Erythropoietin (EPO); EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin; EPO protein

• Host: 293F cell
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 193

• Applicable Applications for EPO protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Ala28~Arg193
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Eukaryotic expression
• Organism Species: Human
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 8.8
• Subcellular Location: Secreted
• Usage: Reconstitute in PBS or others.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

Product Categories/Family for EPO protein

Eukaryotic proteins

NCBI and Uniprot Product Information

• NCBI GI #: 62240997
• NCBI GeneID: 2056
• NCBI Accession #: NP_000790.2
• NCBI GenBank Nucleotide #: NM_000799.2
• UniProt Accession #: P01588; Q2M2L6; Q549U2; Q9UDZ0; Q9UEZ5; Q9UHA0
• Molecular Weight: 20kDa; 20kDa
• NCBI Official Full Name: erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: EPO
• NCBI Official Synonym Symbols: EP; MVCD2
• NCBI Protein Information: erythropoietin
• UniProt Protein Name: Erythropoietin
• Protein Family: Erythropoietin
• UniProt Gene Name: EPO

NCBI Description

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]

Uniprot Description

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: cell surface; extracellular region; extracellular space

Molecular Function: protein binding

Biological Process: erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of Ras protein signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of Stat5 protein

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2