Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) Protein | GMCSF protein

Eukaryotic Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

Cat. Num. AAA2086663

• Gene Names: CSF2; GMCSF
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Colony Stimulating Factor 2; Granulocyte Macrophage (GMCSF); Eukaryotic Colony Stimulating Factor 2; CSF2; GM-CSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor; GMCSF protein

• Host: 293F cell
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 144

• Applicable Applications for GMCSF protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Ala18~Glu144
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Eukaryotic expression
• Organism Species: Human
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 5.2
• Subcellular Location: Secreted
• Usage: Reconstitute in PBS or others.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Product Categories/Family for GMCSF protein

Eukaryotic proteins

NCBI and Uniprot Product Information

• NCBI GI #: 27437030
• NCBI GeneID: 1437
• NCBI Accession #: NP_000749.2
• NCBI GenBank Nucleotide #: NM_000758.3
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6
• Molecular Weight: 16.1kDa; 16kDa
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular region

Molecular Function: protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: MAPKKK cascade; myeloid dendritic cell differentiation; negative regulation of cytolysis; positive regulation of DNA replication; positive regulation of interleukin-23 production; positive regulation of tyrosine phosphorylation of Stat5 protein