Angiotensinogen (AGT) Protein | AGT protein

Eukaryotic Angiotensinogen (AGT)

Cat. Num. AAA2086722

• Gene Names: Agt; Ang; PAT; ANRT; AngII
• Applications: SDS-Page, Western Blot
• Purity: > 97%
• Synonyms: Angiotensinogen (AGT); Eukaryotic Angiotensinogen (AGT); ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor; Clade A; Member 8(Alpha-1 Antiproteinase; Antitrypsin); AGT protein

• Host: 293F cell
• Purity/Purification: > 97%
• Form/Format: Freeze-dried powder
• Sequence Length: 477

• Applicable Applications for AGT protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Asp25~Val477
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Eukaryotic expression
• Organism Species: Rat
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 5.3
• Subcellular Location: Secreted
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Testing Data

Product Categories/Family for AGT protein

Eukaryotic proteins

NCBI and Uniprot Product Information

• NCBI GI #: 19705570
• NCBI GeneID: 24179
• NCBI Accession #: NP_602308.1
• NCBI GenBank Nucleotide #: NM_134432.2
• UniProt Accession #: P01015
• Molecular Weight: 51.2kDa; 60kDa
• NCBI Official Full Name: angiotensinogen
• NCBI Official Synonym Full Names: angiotensinogen
• NCBI Official Symbol: Agt
• NCBI Official Synonym Symbols: Ang; PAT; ANRT; AngII
• NCBI Protein Information: angiotensinogen
• UniProt Protein Name: Angiotensinogen
• Protein Family: Angiotensinogen
• UniProt Gene Name: Agt
• UniProt Synonym Gene Names: Serpina8; Ang I; Ang II; Ang III; Ang IV

NCBI Description

plays a role in regulation of blood pressure [RGD, Feb 2006]

Uniprot Description

angiotensin: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1- 8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2. Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT). Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause. Defects in AGT are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: cytoplasm; extracellular space; mitochondrion

Molecular Function: angiotensin receptor binding; hormone activity; receptor agonist activity; serine-type endopeptidase inhibitor activity; sodium channel regulator activity; type 1 angiotensin receptor binding; type 2 angiotensin receptor binding

Biological Process: activation of NF-kappaB-inducing kinase; aging; angiotensin mediated drinking behavior; angiotensin mediated regulation of renal output; angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure; artery smooth muscle contraction; astrocyte activation; blood vessel development; brain renin-angiotensin system; cell surface receptor linked signal transduction; cell-matrix adhesion; cellular sodium ion homeostasis; cytokine secretion; drinking behavior; elevation of cytosolic calcium ion concentration; establishment of blood-nerve barrier; excretion; extracellular matrix organization and biogenesis; female pregnancy; fibroblast proliferation; G-protein coupled receptor protein signaling pathway; hormone metabolic process; kidney development; negative regulation of angiogenesis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of nerve growth factor receptor signaling pathway; negative regulation of neuron apoptosis; negative regulation of tissue remodeling; ovarian follicle rupture; peristalsis; phospholipase C activation; positive regulation of blood pressure; positive regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of cellular protein metabolic process; positive regulation of cytokine secretion; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of fatty acid biosynthetic process; positive regulation of fibroblast proliferation; positive regulation of MAPKKK cascade; positive regulation of multicellular organism growth; positive regulation of nitric oxide biosynthetic process; positive regulation of organ growth; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of superoxide release; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; regulation of apoptosis; regulation of blood pressure; regulation of calcium ion transport; regulation of gene expression; regulation of heart rate; regulation of inflammatory response; regulation of long-term neuronal synaptic plasticity; regulation of norepinephrine secretion; regulation of systemic arterial blood pressure by circulatory renin-angiotensin; regulation of transmission of nerve impulse; renal response to blood flow during renin-angiotensin regulation of systemic arterial blood pressure; renal system process; renin-angiotensin regulation of aldosterone production; response to cold; response to muscle activity involved in regulation of muscle adaptation; response to salt stress; smooth muscle cell differentiation; smooth muscle cell proliferation; stress-activated MAPK cascade; ureteric bud branching; vasoconstriction; vasodilation