Plasmin/Antiplasmin Complex (PAP) Protein | PAP protein
Eukaryotic Plasmin/Antiplasmin Complex (PAP)
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification:
Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
NCBI and Uniprot Product Information
Accurate Molecular Mass: 120kDa as determined by SDS-PAGE reducing conditions.
NCBI Description
This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Uniprot Description
SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 17p13
Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; protease binding; endopeptidase inhibitor activity
Biological Process: platelet activation; collagen fibril organization; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; response to organic substance; fibrinolysis; platelet degranulation; negative regulation of fibrinolysis; renin-angiotensin regulation of blood vessel size; positive regulation of stress fiber formation; acute-phase response; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; blood coagulation
Disease: Alpha-2-plasmin Inhibitor Deficiency
