Activity Data
(Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL6 induces C-reactive protein (CRP) expression through the STAT3 pathway in HepG2 cells. To test this effect, HepG2 cells were seeded overnight at a density of 1x10^5 cells/mL, and treated with or without various concentrations of IL6 for 24h and CRP levels in the cell supernatant were determined by ELISA.CRP levels in the cell supernatant of HepG2 cells increased significantly after stimulated with IL6, the data was shown in Table 1 and Figure 1.)
Interleukin 6 (IL6) Active Protein | IL6 active protein
Active Interleukin 6 (IL6)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Activity Data
(Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL6 induces C-reactive protein (CRP) expression through the STAT3 pathway in HepG2 cells. To test this effect, HepG2 cells were seeded overnight at a density of 1x10^5 cells/mL, and treated with or without various concentrations of IL6 for 24h and CRP levels in the cell supernatant were determined by ELISA.CRP levels in the cell supernatant of HepG2 cells increased significantly after stimulated with IL6, the data was shown in Table 1 and Figure 1.)
Activity Data
(Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL6 induces C-reactive protein (CRP) expression through the STAT3 pathway in HepG2 cells. To test this effect, HepG2 cells were seeded overnight at a density of 1x10^5 cells/mL, and treated with or without various concentrations of IL6 for 24h and CRP levels in the cell supernatant were determined by ELISA.CRP levels in the cell supernatant of HepG2 cells increased significantly after stimulated with IL6, the data was shown in Table 1 and Figure 1.)
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Testing Data
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NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the interleukin family of cytokines that have important functions in immune response, hematopoiesis, inflammation and the acute phase response. The ectopic overexpression of the encoded protein in mice results in excessive plasma cells in circulation, leading to death. Mice lacking the encoded protein exhibit abnormalities in hepatic acute phase response, some immune mechanisms, bone resorption in response to estrogen, liver regeneration and wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
Uniprot Description
IL6: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic- onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Belongs to the IL-6 superfamily.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5 B1|5 15.7 cM
Cellular Component: cytoplasm; external side of plasma membrane; extracellular space; interleukin-6 receptor complex
Molecular Function: cytokine activity; growth factor activity; interleukin-6 receptor binding; protein binding; receptor binding
Biological Process: cell growth; cell redox homeostasis; cytokine and chemokine mediated signaling pathway; defense response to protozoan; defense response to virus; endocrine pancreas development; glucose homeostasis; hepatic immune response; inflammatory response; muscle maintenance; myeloid cell homeostasis; negative regulation of apoptosis; negative regulation of bone resorption; negative regulation of caspase activity; negative regulation of cell proliferation; negative regulation of chemokine biosynthetic process; negative regulation of collagen biosynthetic process; negative regulation of cytokine secretion; negative regulation of gluconeogenesis; negative regulation of hormone secretion; negative regulation of membrane potential; negative regulation of muscle development; negative regulation of protein kinase activity; neurite development; neutrophil apoptosis; positive regulation of acute inflammatory response; positive regulation of apoptosis; positive regulation of B cell activation; positive regulation of cell proliferation; positive regulation of chemokine production; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of immunoglobulin secretion; positive regulation of interleukin-6 production; positive regulation of JAK-STAT cascade; positive regulation of MAPKKK cascade; positive regulation of neuron differentiation; positive regulation of nitric oxide biosynthetic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein import into nucleus, translocation; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell proliferation; positive regulation of T cell proliferation; positive regulation of T-helper 2 cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of translation; positive regulation of transmission of nerve impulse; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of apoptosis; regulation of cell proliferation; regulation of cell shape; regulation of circadian sleep/wake cycle, non-REM sleep; response to glucocorticoid stimulus; response to wounding; T cell activation
Research Articles on IL6
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Product Notes
The IL6 il6 (Catalog #AAA2086067) is an Active Protein produced from 293F cell and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interleukin 6 (IL6) can be used in a range of immunoassay formats including, but not limited to, Cell culture, Activity Assays. Researchers should empirically determine the suitability of the IL6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 6 (IL6), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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