Zona pellucida sperm-binding protein 3 (ZP3) Recombinant Protein | ZP3 recombinant protein

Recombinant Dog Zona pellucida sperm-binding protein 3 (ZP3)

Cat. Num. AAA964940

• Gene Names: ZP3; ZPC; Zp-3
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Zona pellucida sperm-binding protein 3 (ZP3); Recombinant Dog Zona pellucida sperm-binding protein 3 (ZP3); Recombinant Zona pellucida sperm-binding protein 3 (ZP3); Zona pellucida sperm-binding protein 3; Sperm receptor Zona pellucida glycoprotein 3; Zp-3 Zona pellucida protein C Cleaved into the following chain: 1. Processed zona pellucida sperm-binding prote; ZP3 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 23-350. Full length of the mature protein (Provide the Zona pellucida sperm-binding protein 3 chain)
• Sequence: QTIWPTETYYPLTSRPPVMVDCLESQLVVTVSKDLFGTGKLIRPADLTLGPENCEPLVSMDTDDVVRFEVGLHECGSRVQVTDNALVYSTFLIHSPRPAGNLSILRTNRAEVPIECHYPRHSNVSSQAILPTWVPFRTTMLFEEKLVFSLRLMEEDWGSEKQSPTFQLGDIAHLQAEVHTGSHMPLRLFVDHCVATLTPDRNAFLHHKIVDFHGCLVDGLYNSSSAFKAPRPRPETLQFTVDVFHFAKDSRNTIYITCHLKVTPADRVPDQLNKACSFIKSTKRWYPVEGSADICRCCNKGSCGLPGRSRRLSHLERGWRKSVSHTRN
• Sequence Length: 350

• Species: Canis familiaris (Dog) (Canis lupus familiaris)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Related Product Information for ZP3 recombinant protein

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. This protein is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 50979000
• NCBI GeneID: 403894
• NCBI Accession #: NP_001003224.1
• NCBI GenBank Nucleotide #: NM_001003224.1
• UniProt Accession #: P48831
• Molecular Weight: 47,368 Da
• NCBI Official Full Name: zona pellucida sperm-binding protein 3
• NCBI Official Symbol: ZP3
• NCBI Official Synonym Symbols: ZPC; Zp-3
• NCBI Protein Information: zona pellucida sperm-binding protein 3; sperm receptor; zona pellucida protein C; zona pellucida glycoprotein ZP3; zona pellucida 3 glycoprotein (sperm receptor)
• UniProt Protein Name: Zona pellucida sperm-binding protein 3
• Protein Family: Zona pellucida sperm-binding protein
• UniProt Gene Name: ZP3
• UniProt Synonym Gene Names: ZPC; Zp-3
• UniProt Entry Name: ZP3_CANFA

Uniprot Description

Function: The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

Subunit structure: Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers

By similarity.

Subcellular location: Processed zona pellucida sperm-binding protein 3: Secreted › extracellular space › extracellular matrix. Cell membrane; Single-pass type I membrane protein.

Tissue specificity: Oocytes.

Domain: The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Post-translational modification: Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.N-glycosylated

By similarity.O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy

By similarity.

Sequence similarities: Belongs to the ZP domain family. ZPC subfamily.Contains 1 ZP domain.