Mouse Zinc finger FYVE domain-containing protein 21 ELISA Kit | ZFYVE21 elisa kit

Mouse Zinc finger FYVE domain-containing protein 21 (ZFYVE21) ELISA Kit

Cat. Num. AAA280476

• Gene Names: Zfyve21; C85416; AW558780; 1110013H04Rik
• Reactivity: Mouse
• Synonyms: Zinc finger FYVE domain-containing protein 21; Mouse Zinc finger FYVE domain-containing protein 21 (ZFYVE21) ELISA Kit; ZFYVE21 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ZFYVE21. No significant cross-reactivity or interference between Mouse ZFYVE21 and analogues was observed.
• Sequence Length: 252

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ZFYVE21 elisa kit

The FYVE domain of ZF21 was required for its membrane localization. Knockdown of ZF21 in human cell lines via small hairpin RNA increased cell adherence to fibronectin (FN1), collagen I, and vitronectin (VTN), but not poly-L-lysine, and reduced cell motility. Knockdown of ZF21 was associated with enhanced cell surface expression of integrin beta-1 (ITGB1) and increased number of focal adhesions. ZF21 did not affect formation of focal adhesions, but appeared to regulate their disassembly. Protein pull-down analysis showed that ZF21 interacted with the focal adhesion protein FAK (PTK2), and domain analysis revealed that the FYVE domain of ZF21 interacted with the kinase domain FAK.ZF21 dephosphorylated tyr397 of FAK, an event crucial for the induction of focal adhesion disassembly.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZFYVE21 in samples. An antibody specific for ZFYVE21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFYVE21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFYVE21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFYVE21 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 326937469
• NCBI GeneID: 68520
• NCBI Accession #: NP_081028.3
• NCBI GenBank Nucleotide #: NM_026752.4
• UniProt Accession #: Q8VCM3; Q3TBQ9; Q9D1E2; A3KML1
• Molecular Weight: 26,047 Da
• NCBI Official Full Name: zinc finger FYVE domain-containing protein 21
• NCBI Official Synonym Full Names: zinc finger, FYVE domain containing 21
• NCBI Official Symbol: Zfyve21
• NCBI Official Synonym Symbols: C85416; AW558780; 1110013H04Rik
• NCBI Protein Information: zinc finger FYVE domain-containing protein 21
• UniProt Protein Name: Zinc finger FYVE domain-containing protein 21
• Protein Family: Zinc finger FYVE domain-containing protein
• UniProt Gene Name: Zfyve21

Uniprot Description

ZFYVE21: Plays a role in cell adhesion, and thereby in cell motility, regulating microtubule-induced PTK2/FAK1 dephosphorylation, an event important for focal adhesion disassemblya, as well as integrin beta-1/ITGB1 cell surface expression. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 12|12 F1

Cellular Component: cell junction; cytoplasmic vesicle; endosome

Molecular Function: metal ion binding