Mouse Zinc finger protein 161 homolog ELISA Kit | ZFP161 elisa kit
Mouse Zinc finger protein 161 homolog (ZFP161) ELISA Kit
Gene Names
Zbtb14; ZF5; Zfp161; b2b1982Clo
Reactivity
Mouse
Synonyms
Zinc finger protein 161 homolog; Mouse Zinc finger protein 161 homolog (ZFP161) ELISA Kit; ZFP161 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ZFP161. No significant cross-reactivity or interference between Mouse ZFP161 and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZFP161 elisa kit
ZFP161 encodes a zinc finger protein of 449 amino acids with a predicted mass of 51 kD. The deduced amino acid sequence is 99% identical to mouse ZF5 and 95% identical to chicken ZF5. Mouse ZF5 has been shown to be a transcription repressor of c-myc and tk promoters. The ZF5 sequence contains a hydrophobic domain, known as BTB/POZ, near its N terminus. The protein contains 5 Kruppel-type C2H2 zinc fingers near its C terminus.
Sugiura et al. (1997) used immunofluorescence to localize the ZF5 protein in transfected COS-7 cells. Full-length ZF5 protein was located in discrete dots in the nucleus, while ZF5 lacking the BTB/POZ domain was located in the nuclear periphery, suggesting to the authors that the BTB/POZ domain directs the protein to specific sites in the nucleus.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZFP161 in samples. An antibody specific for ZFP161 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFP161 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFP161 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFP161 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sugiura et al. (1997) used immunofluorescence to localize the ZF5 protein in transfected COS-7 cells. Full-length ZF5 protein was located in discrete dots in the nucleus, while ZF5 lacking the BTB/POZ domain was located in the nuclear periphery, suggesting to the authors that the BTB/POZ domain directs the protein to specific sites in the nucleus.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZFP161 in samples. An antibody specific for ZFP161 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFP161 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFP161 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFP161 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,899 Da
NCBI Official Full Name
zinc finger and BTB domain-containing protein 14
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 14
NCBI Official Symbol
Zbtb14
NCBI Official Synonym Symbols
ZF5; Zfp161; b2b1982Clo
NCBI Protein Information
zinc finger and BTB domain-containing protein 14
UniProt Protein Name
Zinc finger and BTB domain-containing protein 14
UniProt Gene Name
Zbtb14
UniProt Synonym Gene Names
Zfp-5; Zfp161; Zfp5; Zfp-161; ZF5
Uniprot Description
ZFP161: Transcriptional repressor of MYC and thymidine kinase promoters. Transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 17 E1.3|17 40.42 cM
Cellular Component: cytosol; nucleolus; nucleoplasm; nucleus
Molecular Function: DNA binding; DNA binding transcription factor activity; metal ion binding; nucleic acid binding; sequence-specific DNA binding
Biological Process: negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of transcription, DNA-templated; transcription, DNA-dependent
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Product Notes
The Mouse ZFP161 zbtb14 (Catalog #AAA281907) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281907 ELISA Kit recognizes Mouse ZFP161. It is sometimes possible for the material contained within the vial of "Zinc finger protein 161 homolog, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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