Mouse Zinc finger E-box-binding homeobox 2 ELISA Kit | ZEB2 elisa kit
Mouse Zinc finger E-box-binding homeobox 2 (ZEB2) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Delta-Ef1 selectively transactivated the promoters of the SMC differentiation marker genes Acta2 and Myh11, and it physically interacted with Srf and Smad3, resulting in synergistic activation of the Acta2 promoter. Chromatin immunoprecipitation assays and knockdown experiments showed that delta-Ef1 was involved in control of SMC differentiation programs induced by Tgf-beta (TGFB1) signaling. Overexpression of delta-Ef1 inhibited neointima formation and promoted SMC differentiation.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZEB2 in samples. An antibody specific for ZEB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZEB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZEB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZEB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
Uniprot Description
ZEB2: Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin. Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS); also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Protein type: C2H2-type zinc finger protein; DNA-binding; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 2|2 B
Cellular Component: cytosol; nuclear chromatin; nucleolus; nucleus
Molecular Function: DNA binding; metal ion binding; nucleic acid binding; sequence-specific DNA binding
Biological Process: cell proliferation in forebrain; central nervous system development; collateral sprouting; corpus callosum morphogenesis; corticospinal tract morphogenesis; embryonic morphogenesis; hippocampus development; negative regulation of transcription from RNA polymerase II promoter; neural crest cell migration; neural tube closure; pigmentation during development; positive regulation of axonogenesis; positive regulation of JUN kinase activity; positive regulation of melanin biosynthetic process; positive regulation of melanocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; regulation of transcription, DNA-templated; somitogenesis; transcription, DNA-dependent
Research Articles on ZEB2
Similar Products
Product Notes
The Mouse ZEB2 zeb2 (Catalog #AAA283408) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283408 ELISA Kit recognizes Mouse ZEB2. It is sometimes possible for the material contained within the vial of "Zinc finger E-box-binding homeobox 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
