Mouse Zinc finger and BTB domain-containing protein 7A ELISA Kit | ZBTB7A elisa kit

Mouse Zinc finger and BTB domain-containing protein 7A (ZBTB7A) ELISA Kit

Cat. Num. AAA282240

• Gene Names: Zbtb7a; Lrf; FBI-1; Zbtb7; Pokemon; AI452336; 9030619K07Rik; 9130006G12Rik
• Reactivity: Mouse
• Synonyms: Zinc finger and BTB domain-containing protein 7A; Mouse Zinc finger and BTB domain-containing protein 7A (ZBTB7A) ELISA Kit; ZBTB7A elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ZBTB7A. No significant cross-reactivity or interference between Mouse ZBTB7A and analogues was observed.
• Sequence Length: 584

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ZBTB7A elisa kit

LRF interacted with BCL6 but not with PLZF. The interaction required both the BTB/POZ and zinc finger domains of the 2 proteins. Confocal microscopy demonstrated colocalization of LRF and BCL6 in the nucleus.BTB/POZ domains can form homomeric or heterodimeric complexes and are found in some viral and cellular proteins that may not be associated with transcription. The mouse and human LRF proteins share 88% amino acid identity. Northern blot analysis revealed wide expression of 3 transcripts in various cell lines as well as in skin, lung, and liver, but not in spleen or thymus. Whole mount in situ hybridization analysis showed expression of Lrf in the first 2 pharyngeal arches, limb buds, tail buds, and the neural tube of mouse embryos, suggesting a role in stimulating cell proliferation.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZBTB7A in samples. An antibody specific for ZBTB7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZBTB7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZBTB7A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZBTB7A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 117647238
• NCBI GeneID: 16969
• NCBI Accession #: NP_034861.3
• NCBI GenBank Nucleotide #: NM_010731.3
• UniProt Accession #: O88939; Q3U372; Q9CRJ0; B2RRP7; B7ZNL6
• Molecular Weight: 59,778 Da
• NCBI Official Full Name: zinc finger and BTB domain-containing protein 7A
• NCBI Official Synonym Full Names: zinc finger and BTB domain containing 7a
• NCBI Official Symbol: Zbtb7a
• NCBI Official Synonym Symbols: Lrf; FBI-1; Zbtb7; Pokemon; AI452336; 9030619K07Rik; 9130006G12Rik
• NCBI Protein Information: zinc finger and BTB domain-containing protein 7A
• UniProt Protein Name: Zinc finger and BTB domain-containing protein 7A
• Protein Family: Zinc finger and BTB domain-containing protein
• UniProt Gene Name: Zbtb7a
• UniProt Synonym Gene Names: Lrf; Zbtb7; POK erythroid myeloid ontogenic factor; Pokemon

Uniprot Description

FBI1: a widely expressed zinc finger protein. Apparently interacts with Bcl-6.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 10|10 C1

Cellular Component: nucleus

Molecular Function: DNA binding; DNA binding transcription factor activity; histone acetyltransferase binding; metal ion binding; nucleic acid binding; protein binding

Biological Process: cartilage development; cell differentiation; multicellular organism development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of osteoclast differentiation; regulation of transcription, DNA-templated; transcription, DNA-dependent