Mouse Zinc finger and BTB domain-containing protein 32 ELISA Kit | ZBTB32 elisa kit

Mouse Zinc finger and BTB domain-containing protein 32 (ZBTB32) ELISA Kit

Cat. Num. AAA282386

• Gene Names: Zbtb32; Rog; FAXF; FAZF; PLZP; Tzfp; 4930524C15Rik
• Reactivity: Mouse
• Synonyms: Zinc finger and BTB domain-containing protein 32; Mouse Zinc finger and BTB domain-containing protein 32 (ZBTB32) ELISA Kit; ZBTB32 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ZBTB32. No significant cross-reactivity or interference between Mouse ZBTB32 and analogues was observed.
• Sequence Length: 487

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ZBTB32 elisa kit

Sequence analysis predicted that the 487-amino acid protein, which is similar to PLZF, contains an N-terminal POZ/BTB domain and 3 zinc fingers. Northern blot analysis detected weak expression of a 1.4-kb transcript in most tissues tested except peripheral blood leukocytes, where strong expression was detected, and testis, where strong expression of 2.0-, 2.7-, and 4.4-kb transcripts was detected. Coimmunoprecipitation analysis showed that FAZF associates with FANCC independent of the BTB/POZ domain. FANCC and PLZF did not colocalize. EMSA analysis suggested that PLZF and FAZF bind to CpG islands, interact with each other through the POZ domain of PLZF, and repress transcription by a similar mechanism.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZBTB32 in samples. An antibody specific for ZBTB32 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZBTB32 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZBTB32 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZBTB32 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 134032011
• NCBI GeneID: 58206
• NCBI Accession #: NP_067372.2
• NCBI GenBank Nucleotide #: NM_021397.2
• UniProt Accession #: Q9JKD9; Q9D523
• Molecular Weight: 11,021 Da
• NCBI Official Full Name: zinc finger and BTB domain-containing protein 32
• NCBI Official Synonym Full Names: zinc finger and BTB domain containing 32
• NCBI Official Symbol: Zbtb32
• NCBI Official Synonym Symbols: Rog; FAXF; FAZF; PLZP; Tzfp; 4930524C15Rik
• NCBI Protein Information: zinc finger and BTB domain-containing protein 32
• UniProt Protein Name: Zinc finger and BTB domain-containing protein 32
• Protein Family: Zinc finger and BTB domain-containing protein
• UniProt Gene Name: Zbtb32
• UniProt Synonym Gene Names: Rog; Tzfp

Uniprot Description

ZBTB32: DNA-binding protein that binds to the to a 5'- TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 7|7 B1

Cellular Component: nuclear chromosome; nucleus

Molecular Function: DNA binding; metal ion binding; nucleic acid binding; transcription corepressor activity; zinc ion binding

Biological Process: hemopoiesis; regulation of cytokine production; regulation of transcription, DNA-templated; T cell proliferation; transcription, DNA-dependent