ZBTB17 recombinant protein

Recombinant Human ZBTB17 Protein

Cat. Num. AAA8250039

• Gene Names: ZBTB17; MIZ-1; ZNF60; ZNF151; pHZ-67
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: ZBTB17; Recombinant Human ZBTB17 Protein; MIZ1; ZNF151; ZNF60; Zinc finger and BTB domain-containing protein 17; Myc-interacting zinc finger protein 1; Miz-1; Zinc finger protein 151; Zinc finger protein 60; ZBTB17 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.25.
• Sequence: MGSSHHHHHH SSGLVPRGSH MDFPQHSQHV LEQLNQQRQL GLLCDCTFVV DGVHFKAHKA VLAACSEYFK MLFVDQKDVV HLDISNAAGL GQVLEFMYTA KLSLSPENVD DVLAVATFLQ MQDIITACHA LKSLAEPATS PGGNAEALAT EGGDKRAKEE KVATSTLSRL EQAGRSTPIG PSRDLKEERG GQAQSAASGA EQTEKADA
• Sequence Length: 721

• Applicable Applications for ZBTB17 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for ZBTB17 recombinant protein

Recombinant Human ZBTB17 Protein is produced by our E.coli expression system and the target gene encoding Met1-Ala188 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 338968907
• NCBI GeneID: 7709
• NCBI Accession #: NP_001229813.1
• NCBI GenBank Nucleotide #: NM_001242884.1
• UniProt Accession #: Q13105; Q15932; Q5JYB2; Q9NUC9; A0AV07; B4DXB4; B7ZLQ9; F5H411
• Molecular Weight: 79,228 Da
• NCBI Official Full Name: zinc finger and BTB domain-containing protein 17 isoform 1
• NCBI Official Synonym Full Names: zinc finger and BTB domain containing 17
• NCBI Official Symbol: ZBTB17
• NCBI Official Synonym Symbols: MIZ-1; ZNF60; ZNF151; pHZ-67
• NCBI Protein Information: zinc finger and BTB domain-containing protein 17
• UniProt Protein Name: Zinc finger and BTB domain-containing protein 17
• Protein Family: Zinc finger and BTB domain-containing protein
• UniProt Gene Name: ZBTB17
• UniProt Synonym Gene Names: MIZ1; ZNF151; ZNF60; Miz-1
• UniProt Entry Name: ZBT17_HUMAN

NCBI Description

This gene encodes a zinc finger protein involved in the regulation of c-myc. The symbol MIZ1 has also been associated with PIAS2 which is a different gene located on chromosome 18. [provided by RefSeq, Jul 2008]

Uniprot Description

ZBTB17: a transcription factor that can function as an activator or repressor depending on its binding partners, and by targeting negative regulators of cell cycle progression. Plays a critical role in early lymphocyte development, where it is essential to prevent apoptosis in lymphoid precursors, allowing them to survive in response to IL7 and undergo proper lineage commitment. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Required for early embryonic development during gastrulation. Represses RB1 transcription; this repression can be blocked by interaction with ZBTB49 isoform 3/ZNF509S1. Belongs to the krueppel C2H2-type zinc-finger protein family. Binds to the C-terminal helix-loop-helix motif of MYC which inhibits ZBTB17 transactivation and growth arrest activities and renders it insoluble in the nucleus. Also interacts with HCFC1, MAGEA4 and TMPRSS11A. Three isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: nucleoplasm

Molecular Function: protein binding; transcription factor activity

Biological Process: negative regulation of cell cycle