Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) Recombinant Protein | YWHAz recombinant protein

Recombinant Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)

Cat. Num. AAA2009082

• Gene Names: YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz); Recombinant Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz); YWHAz recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-MDKNELV QKAKLAEQAE RYDDMAACMK SVTEQGAELS NEERNLLSVA YKNVVGARRS SWRVVSSIEQ KTEGAEKKQQ MAREYREKIE TELRDICNDV LSLLEKFLIP NASQAESKVF YLKMKGDYYR YLAEVAAGDD KKGIVDQSQQ AYQEAFEISK KEMQPTHPIR LGLALNFSVF YYEILNSPEK ACSLAKTAFD EAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDTQGDEA EAGEGGEN
• Sequence Length: 245

• Applicable Applications for YWHAz recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Met1~Asn245 (Accession # P63104) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 208973238
• NCBI GeneID: 7534
• NCBI Accession #: NP_001129171.1
• NCBI GenBank Nucleotide #: NM_001135699.1
• UniProt Accession #: P63104; P29213; P29312; Q32P43; Q5XJ08; Q6GPI2; Q6IN74; Q6NUR9; Q6P3U9; Q86V33; A8K1N0; B7Z465
• Molecular Weight: 29.3kDa
• NCBI Official Full Name: 14-3-3 protein zeta/delta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
• NCBI Official Symbol: YWHAZ
• NCBI Official Synonym Symbols: HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta
• NCBI Protein Information: 14-3-3 protein zeta/delta; 14-3-3 zeta; 14-3-3 delta; phospholipase A2; epididymis luminal protein 4; epididymis secretory protein Li 3; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; 14-3-3 protein/cytosolic phospholipase A2; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide
• UniProt Protein Name: 14-3-3 protein zeta/delta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAZ
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433Z_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; cytoplasmic vesicle membrane; mast cell granule; focal adhesion; protein complex; mitochondrion; postsynaptic density; leading edge; cytosol; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; melanosome; nucleus

Molecular Function: identical protein binding; protein domain specific binding; protein binding; protein complex binding; protein kinase binding; transcription factor binding

Biological Process: response to drug; platelet activation; histamine secretion by mast cell; establishment of Golgi localization; apoptosis; gene expression; signal transduction; blood coagulation; protein targeting to mitochondrion; negative regulation of apoptosis