YOD1 recombinant protein

Recombinant Human YOD1

Cat. Num. AAA146613

• Gene Names: YOD1; DUBA8; OTUD2; PRO0907
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: YOD1; Recombinant Human YOD1; YOD1 Human; YOD1 Human Recombinant; DUBA8; OTUD2; PRO0907; RP11-164O23.1; Ubiquitin thioesterase OTU1; DUBA-8; HIN-7; HsHIN7; OTU domain-containing protein 2; YOD1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The YOD1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMFGPAKG RHFGVHPAPG FPGGVSQQAA GTKAGPAGAW PVGSRTDTMW RLRCKAKDGT HVLQGLSSRT RVRELQGQIA AITGIAPGGQ RILVGYPPEC LDLSNGDTIL EDLPIQSGDM LIIEEDQTRP RSSPAFTKRG ASSYVRETLP VLTRTVVPAD NSCLFTSVYY VVEGGVLNPA CAPEMRRLIA QIVASDPDFY SEAILGKTNQ EYCDWIKRDD TWGGAIEISI LSKFYQCEIC VVDTQTVRID RFGEDAGYTK RVLLIYDGIH YDPLQRNFPD PDTPPLTIFS SNDDIVLVQA LELADEARRR RQFTDVNRFT LRCMVCQKGL TGQAEAREHA KETGHTNFGE V
• Sequence Length: 304

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for YOD1 recombinant protein

Description: YOD1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 371 amino acids (1-348) and having a molecular mass of 40.7kDa.YOD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: YOD1 is a Hydrolase which removes conjugated ubiquitin from proteins and takes part in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. YOD1 is a highly conserved deubiquitinating enzyme belonging to the ovarian tumor (otubain) family, whose function has yet to be determined in mammalian cells. YOD1 is a component of a multiprotein complex with p97 as its nucleus, proposing a functional link to a pathway responsible for the dislocation of misfolded proteins from the endoplasmic reticulum. YOD1 variant xpression deprived of its deubiquitinating activity compels a halt on the dislocation reaction, as concluded by the stabilization of various dislocation substrates.

Product Categories/Family for YOD1 recombinant protein

Enzymes; Esterase

NCBI and Uniprot Product Information

• NCBI GI #: 444299626
• NCBI GeneID: 55432
• NCBI Accession #: NP_001263249.1
• NCBI GenBank Nucleotide #: NM_001276320.1
• UniProt Accession #: Q5VVQ6; Q5VVQ5; Q6ZRS6; Q86T63; Q9P1L8; B2RNX3
• Molecular Weight: 33,979 Da
• NCBI Official Full Name: ubiquitin thioesterase OTU1 isoform 2
• NCBI Official Synonym Full Names: YOD1 deubiquitinase
• NCBI Official Symbol: YOD1
• NCBI Official Synonym Symbols: DUBA8; OTUD2; PRO0907
• NCBI Protein Information: ubiquitin thioesterase OTU1; DUBA-8; HIN-7; HIV-1-induced protease 7; OTU domain containing 2; OTU domain-containing protein 2; YOD1 OTU deubiquinating enzyme 1 homolog ( yeast); hsHIN7
• UniProt Protein Name: Ubiquitin thioesterase OTU1
• UniProt Gene Name: YOD1
• UniProt Synonym Gene Names: DUBA8; HIN7; OTUD2; HIN-7; HsHIN7
• UniProt Entry Name: OTU1_HUMAN

NCBI Description

Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. The protein encoded by this gene belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

YOD1: Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di- ubiquitin. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - unsaturated fatty acid biosynthesis; Secondary Metabolites Metabolism - limonene and pinene degradation; Protease; C2H2-type zinc finger protein; Ubiquitin-specific protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 1q32.2

Molecular Function: protein binding; metal ion binding; ubiquitin-specific protease activity

Biological Process: ER-associated protein catabolic process; unfolded protein response