DNA adenine methyltransferase YhdJ (yhdJ) Recombinant Protein | yhdJ recombinant protein
Recombinant Escherichia coli DNA adenine methyltransferase YhdJ (yhdJ)
NCBI and Uniprot Product Information
NCBI Description
In vitro DNA adenine methyltransferase activity has been demonstrated for YhdJ; overexpression of YhdJ leads to protection of chromosomal DNA from NsiI digestion, but DNA from wild type cells is not protected (Broadbent (2007). [More information is available at EcoGene: EG11498]. Overexpression of YdhJ leads to methylation of genomic DNA at the NsiI recognition sequence (5'-ATGCAT-3'), and partially purified YdhJ is shown to methylate this sequence to produce N6-methyladenine in the 3' adenine position of ATGCAT in vitro . ydhJ is not an essential gene, and overexpression of ydhJ does not alter cell morphology . [More information is available at EcoCyc: EG11498].
Uniprot Description
Methylates the second adenine of the NsiI recognition sequence (5'-ATGCAT-3').
Research Articles on yhdJ
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Product Notes
The yhdJ yhdj (Catalog #AAA1015932) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-294, full length protein. The amino acid sequence is listed below: MRTGCEPTRF GNEAKTIIHG DALAELKKIP AESVDLIFAD PPYNIGKNFD GLIEAWKEDL FIDWLFEVIA ECHRVLKKQG SMYIMNSTEN MPFIDLQCRK LFTIKSRIVW SYDSSGVQAK KHYGSMYEPI LMMVKDAKNY TFNGDAILVE AKTGSQRALI DYRKNPPQPY NHQKVPGNVW DFPRVRYLMD EYENHPTQKP EALLKRIILA SSNPGDIVLD PFAGSFTTGA VAIASGRKFI GIEINSEYIK MGLRRLDVAS HYSAEELAKV KKRKTGNLSK RSRLSEVDPD LITK. It is sometimes possible for the material contained within the vial of "DNA adenine methyltransferase YhdJ (yhdJ), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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