Macrophage Inflammatory Protein 1 Alpha (MIP1a) Protein | MIP1a protein

Eukaryotic Macrophage Inflammatory Protein 1 Alpha (MIP1a)

Cat. Num. AAA2086689

• Gene Names: CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Macrophage Inflammatory Protein 1 Alpha (MIP1a); Eukaryotic Macrophage Inflammatory Protein 1 Alpha (MIP1a); CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein; MIP1a protein

• Host: Yeast
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 92

• Applicable Applications for MIP1a protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Ser24~Ala92
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Eukaryotic expression
• Organism Species: Human
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 4.8
• Subcellular Location: Secreted
• Usage: Reconstitute in PBS or others.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

Product Categories/Family for MIP1a protein

Eukaryotic proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4506843
• NCBI GeneID: 6348
• NCBI Accession #: NP_002974.1
• NCBI GenBank Nucleotide #: NM_002983.2
• UniProt Accession #: P10147
• Molecular Weight: 9.1kDa; 9kDa
• NCBI Official Full Name: C-C motif chemokine 3
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 3
• NCBI Official Symbol: CCL3
• NCBI Official Synonym Symbols: MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
• NCBI Protein Information: C-C motif chemokine 3
• UniProt Protein Name: C-C motif chemokine 3
• Protein Family: B-box domain protein
• UniProt Gene Name: CCL3
• UniProt Synonym Gene Names: G0S19-1; MIP1A; SCYA3; MIP-1-alpha

NCBI Description

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]

Uniprot Description

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cytoplasm; cytosol; extracellular region; extracellular space; intracellular

Molecular Function: calcium-dependent protein kinase C activity; CCR1 chemokine receptor binding; CCR5 chemokine receptor binding; chemoattractant activity; chemokine activity; identical protein binding; kinase activity; phospholipase activator activity; protein binding; protein kinase activity

Biological Process: astrocyte cell migration; behavior; calcium ion transport; calcium-mediated signaling; cell activation; cell-cell signaling; cellular calcium ion homeostasis; chemotaxis; cytoskeleton organization and biogenesis; eosinophil chemotaxis; eosinophil degranulation; exocytosis; G-protein coupled receptor protein signaling pathway; inflammatory response; lipopolysaccharide-mediated signaling pathway; lymphocyte chemotaxis; macrophage chemotaxis; MAPKKK cascade; monocyte chemotaxis; negative regulation of bone mineralization; negative regulation of osteoclast differentiation; neutrophil chemotaxis; osteoblast differentiation; positive regulation of calcium ion transport; positive regulation of calcium-mediated signaling; positive regulation of cell migration; positive regulation of GTPase activity; positive regulation of inflammatory response; positive regulation of interleukin-1 beta secretion; positive regulation of neuron apoptosis; positive regulation of protein kinase B signaling cascade; positive regulation of tumor necrosis factor production; protein kinase B signaling cascade; regulation of cell shape; regulation of sensory perception of pain; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; response to toxin

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To