Glucose-6-Phosphate Dehydrogenase, Yeast Enzyme | G6PD enzyme

Glucose-6-Phosphate Dehydrogenase, Yeast (G6PDH, G6PD, G6PD1, G6pdx, Glucose 6 phosphate 1 dehydrogenase, MET19, POS10, Zwf1p)

Cat. Num. AAA653987

• Gene Names: G6PD; G6PD1
• Purity: Purified; Purified
• Synonyms: Glucose-6-Phosphate Dehydrogenase; Yeast; Yeast (G6PDH; G6PD; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; MET19; POS10; Zwf1p); G6PD enzyme

• Host: Yeast
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a lyophilized powder from potassium phosphate, sodium citrate and EDTA disodium salt. Ammonium sulfate free.

• Specific Activity: 250 units/mg protein (25 degree C, pH 8.5)
• Contaminants: (as G-6-PDH activity = 100%); Gluutathione reductase: 0.2%; Hexokinase: 0.02%; Phosphogluconate dehydrogenase: 0.01%; Phosphoglucose isomerase: 0.01%; Phosphoglucomutase: 0.01%; Creatine kinase: 0.001%; Myokinase: 0.01%; ATPase: 0.001%
• Preparation and Storage: Long-term storage is recommended at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for G6PD enzyme

Molecular Biology; MB-Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 452269
• NCBI GeneID: 2539
• UniProt Accession #: P11413; Q16000; Q16765; Q8IU70; Q8IU88; Q8IUA6; Q96PQ2; D3DWX9
• Molecular Weight: 59,257 Da
• NCBI Official Full Name: glucose-6-phosphate dehydrogenase
• NCBI Official Synonym Full Names: glucose-6-phosphate dehydrogenase
• NCBI Official Symbol: G6PD
• NCBI Official Synonym Symbols: G6PD1
• NCBI Protein Information: glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase, G6PD
• UniProt Protein Name: Glucose-6-phosphate 1-dehydrogenase
• UniProt Gene Name: G6PD
• UniProt Synonym Gene Names: G6PD
• UniProt Entry Name: G6PD_HUMAN

NCBI Description

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. Ref.28

Catalytic activity: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. Ref.28

Pathway: Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Subunit structure: Homotetramer; dimer of dimers. Ref.25 Ref.28

Tissue specificity: Isoform Long is found in lymphoblasts, granulocytes and sperm.

Polymorphism: The sequence shown is that of variant B, the most common variant. HAMAP-Rule MF_00966

Involvement in disease: Chronic non-spherocytic hemolytic anemia (CNSHA) [MIM:305900]: Deficiency of G6PD is associated with hemolytic anemia in two different situations. First, in areas in which malaria has been endemic, G6PD-deficiency alleles have reached high frequencies (1% to 50%) and deficient individuals, though essentially asymptomatic in the steady state, have a high risk of acute hemolytic attacks. Secondly, sporadic cases of G6PD deficiency occur at a very low frequencies, and they usually present a more severe phenotype. Several types of CNSHA are recognized. Class-I variants are associated with severe NSHA; class-II have an activity <10% of normal; class-III have an activity of 10% to 60% of normal; class-IV have near normal activity.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.32

Miscellaneous: Binds two molecules of NADP. The first one is a cosubstrate (bound to the N-terminal domain), the second is bound to the C-terminal domain and functions as a structural element.

Sequence similarities: Belongs to the glucose-6-phosphate dehydrogenase family.

Biophysicochemical propertiesKinetic parameters:KM=7.07 µM for NADP Ref.28KM=52 µM for glucose 6-phosphate

Sequence caution: The sequence AAA63175.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.