MMP-2 active protein

MMP-2 (catalytic domain) (human), (recombinant)

Cat. Num. AAA566591

• Purity: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Synonyms: MMP-2; MMP-2 (catalytic domain) (human); (recombinant); MMP-2 active protein

• Host: Yeast
• Purity/Purification: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Form/Format: Liquid. In 50mM TRIS-HCl, pH7.5, containing 300mM NaCl, 5mM CaCl2, 20uM ZnCl2, 0.05% Brij-35 and 20% glycerol

• Source Note: Active recombinant matrix metalloproteinase-2 (MMP-2, gelatinase A, 72kDa type IV collagenase) cloned from human cDNA. The enzyme consists of residues Tyr110-Asp452, which comprises the catalytic/fibronectin domain of human MMP-2, with a C-terminal purification tag
• Activity: Preincubation of MMP-2 catalytic domain at 5nM with the broad-spectrum inhibitor GM6001 at 30nM for 1 hour completely inhibits enzymatic activity
• Specific Activity: >=25U/ug. One U=100 pmol/min at 37 degree C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100uM) as substrate
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C; The enzyme is stable on ice for at least several hours. However, it is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. After initial defrost, aliquot product into individual tubes and refreeze at -70 degree C. Avoid repeated freeze/defrost cycles; When stored under the above conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity; Shipping: Shipped on Dry Ice

Related Product Information for MMP-2 active protein

Represents a naturally occurring active form of MMP-2 which lacks the C-terminal hemopexin domain. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected.

Product Categories/Family for MMP-2 active protein

Cellular Analysis; Cell Organelles & Structures; Proteostasis; Proteolysis & Protein Degradation

NCBI and Uniprot Product Information

• UniProt Accession #: P08253
• Molecular Weight: ~40kDa
• UniProt Protein Name: 72 kDa type IV collagenase
• UniProt Gene Name: MMP2
• UniProt Synonym Gene Names: CLG4A; MMP-2
• UniProt Entry Name: MMP2_HUMAN

Uniprot Description

MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family.

Protein type: Cell development/differentiation; Apoptosis; Motility/polarity/chemotaxis; Protease; Secreted, signal peptide; EC 3.4.24.24; Secreted

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: proteinaceous extracellular matrix; extracellular space; sarcomere; mitochondrion; extracellular region; plasma membrane; nucleus

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; serine-type endopeptidase activity

Biological Process: axon guidance; extracellular matrix organization and biogenesis; intramembranous ossification; proteolysis; blood vessel maturation; collagen catabolic process; extracellular matrix disassembly; cellular protein metabolic process; positive regulation of innate immune response; response to hypoxia; ephrin receptor signaling pathway; angiogenesis; embryo implantation

Disease: Multicentric Osteolysis, Nodulosis, And Arthropathy