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SDS-Page

WNT3a recombinant protein

WNT3a, 19-352aa, Human, E Coli (Denatured)

Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
WNT3a; 19-352aa; Human; E Coli (Denatured); Protein Wnt-3a; wnt-3a; WNT3a recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MSYPIWWSLA VGPQYSSLGS QPILCASIPG LVPKQLRFCR NYVEIMPSVA EGIKIGIQEC QHQFRGRRWN CTTVHDSLAI FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTAAICG CSSRHQGSPG KGWKWGGCSE DIEFGGMVSR EFADARENRP DARSAMNRHN NEAGRQAIAS HMHLKCKCHG LSGSCEVKTC WWSQPDFRAI GDFLKDKYDS ASEMVVEKHR ESRGWVETLR PRYTYFKVPT ERDLVYYEAS PNFCEPNPET GSFGTRDRTC NVSSHGIDGC DLLCCGRGHN ARAERRREKC RCVFHWCCYV SCQECTRVYD VHTCK
Sequence Length
352
Applicable Applications for WNT3a recombinant protein
SDS-PAGE
Antigen Species
Human
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for WNT3a recombinant protein
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT3a is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This protein is clustered with WNT14 gene, another family member, in chromosome 1q42 region. Recombinant human WNT3a protein was expressed in E Coli.
Product Categories/Family for WNT3a recombinant protein
References
Baenziger C., et al. (2006). Cell 125:509-522; Bourhis E., et al. (2010). J. Biol. Chem. 285:9172-9179;

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37.5 kDa (335aa)
NCBI Official Full Name
protein Wnt-3a
NCBI Official Synonym Full Names
Wnt family member 3A
NCBI Official Symbol
WNT3A
NCBI Protein Information
protein Wnt-3a
UniProt Protein Name
Protein Wnt-3a
UniProt Gene Name
WNT3A

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

Uniprot Description

Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

Research Articles on WNT3a

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Product Notes

The WNT3a wnt3a (Catalog #AAA205779) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's WNT3a can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the WNT3a wnt3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSYPIWWSLA VGPQYSSLGS QPILCASIPG LVPKQLRFCR NYVEIMPSVA EGIKIGIQEC QHQFRGRRWN CTTVHDSLAI FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTAAICG CSSRHQGSPG KGWKWGGCSE DIEFGGMVSR EFADARENRP DARSAMNRHN NEAGRQAIAS HMHLKCKCHG LSGSCEVKTC WWSQPDFRAI GDFLKDKYDS ASEMVVEKHR ESRGWVETLR PRYTYFKVPT ERDLVYYEAS PNFCEPNPET GSFGTRDRTC NVSSHGIDGC DLLCCGRGHN ARAERRREKC RCVFHWCCYV SCQECTRVYD VHTCK. It is sometimes possible for the material contained within the vial of "WNT3a, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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