Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Mouse WD repeat domain phosphoinositide-interacting protein 1 ELISA Kit | WIPI1 elisa kit

Mouse WD repeat domain phosphoinositide-interacting protein 1 (WIPI1) ELISA Kit

Gene Names
Wipi1; AW411817; D11Ertd498e; 4930533H01Rik
Reactivity
Mouse
Synonyms
WD repeat domain phosphoinositide-interacting protein 1; Mouse WD repeat domain phosphoinositide-interacting protein 1 (WIPI1) ELISA Kit; WIPI1 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse WIPI1. No significant cross-reactivity or interference between Mouse WIPI1 and analogues was observed.
Sequence Length
446
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for WIPI1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WIPI1 in samples. An antibody specific for WIPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWIPI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WIPI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WIPI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,819 Da
NCBI Official Full Name
WD repeat domain phosphoinositide-interacting protein 1
NCBI Official Synonym Full Names
WD repeat domain, phosphoinositide interacting 1
NCBI Official Symbol
Wipi1
NCBI Official Synonym Symbols
AW411817; D11Ertd498e; 4930533H01Rik
NCBI Protein Information
WD repeat domain phosphoinositide-interacting protein 1
UniProt Protein Name
WD repeat domain phosphoinositide-interacting protein 1
UniProt Gene Name
Wipi1
UniProt Synonym Gene Names
D11Ertd498e; WIPI-1; WIPI 49 kDa

Uniprot Description

ATG18: May play a role in autophagy. May regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway. Probably interacts with androgen (AR) and the estrogen receptor (ER). Binds PtdIns3P and to a lesser extent, PtdIns3,5P2 and PtdIns5P in vitro. Interaction with PtdIns3P is required for recruitment to membranes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 11 E1|11 72.18 cM

Cellular Component: cytoplasm; cytoplasmic vesicle; cytoskeleton; cytosol; endosome; endosome membrane; extrinsic component of membrane; Golgi apparatus; membrane; pre-autophagosomal structure membrane; trans-Golgi network

Molecular Function: androgen receptor binding; estrogen receptor binding; phosphatidylinositol 3-phosphate binding; receptor binding

Biological Process: autophagy; mitochondrion degradation; protein lipidation; vesicle targeting, trans-Golgi to endosome

Similar Products

Product Notes

The Mouse WIPI1 wipi1 (Catalog #AAA280657) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280657 ELISA Kit recognizes Mouse WIPI1. It is sometimes possible for the material contained within the vial of "WD repeat domain phosphoinositide-interacting protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.