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SDS-Page

Wnt inhibitory factor 1 Recombinant Protein | Wif1 recombinant protein

Wnt inhibitory factor 1, 29-379aa, Mouse, His tag, Baculovirus

Gene Names
Wif1; WIF-1; AW107799
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
Wnt inhibitory factor 1; 29-379aa; Mouse; His tag; Baculovirus; Wif-1; WIF-1; AW107799; Wif1 recombinant protein
Ordering
For Research Use Only!
Host
Baculovirus
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM MES (pH 5.5) containing 1mM DTT, 1mM PMSF, 30% glycerol.
Concentration
1.0mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
GQPPEESLYL WIDAHQARVL IGFEEDILIV SEGKMAPFTH DFRKAQQRMP AIPVNIHSMN FTWQAAGQAE YFYEFLSLRS LDKGIMADPT VNVPLLGTVP HKASVVQVGF PCLGKQDGVA AFEVNVIVMN SEGNTILRTP QNAIFFKTCQ QAECPGGCRN GGFCNERRVC ECPDGFYGPH CEKALCIPRC MNGGLCVTPG FCICPPGFYG VNCDKANCST TCFNGGTCFY PGKCICPPGL EGEQCELSKC PQPCRNGGKC IGKSKCKCPK GYQGDLCSKP VCEPGCGAHG TCHEPNKCQC REGWHGRHCN KRYGASLMHA PRPAGAGLER HTPSLKKAED RRDPPESNYI WVEHHHHHH
Sequence Length
379
Applicable Applications for Wif1 recombinant protein
SDS-PAGE
Antigen Species
Mouse
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for Wif1 recombinant protein
WIF1, also known as wnt inhibitory factor 1, is a secreted protein that binds to Wnt proteins and inhibits their activities. This protein signaling plays a pivotal role in skeletal development and in the control of cartilage and bone turnover. Also, WIF1 is present in fish, amphibia and mammals, and is expressed during Xenopus and zebrafish development in a complex pattern that includes paraxial presomitic mesoderm, notochord, branchial arches and neural crest derivatives. Recombinant mouse WIF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for Wif1 recombinant protein
References
Park JH., et al. (2014) Dev. Biol. 386 (1):227-236.; Stock M., et al. (2013) Arthritis Rheum. 65 (9):2310-2322.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39.4kDa (359aa) 40-57KDa (SDS-PAGE under reducing conditions.)
NCBI Official Full Name
wnt inhibitory factor 1
NCBI Official Synonym Full Names
Wnt inhibitory factor 1
NCBI Official Symbol
Wif1
NCBI Official Synonym Symbols
WIF-1; AW107799
NCBI Protein Information
wnt inhibitory factor 1
UniProt Protein Name
Wnt inhibitory factor 1
Protein Family
UniProt Gene Name
Wif1
UniProt Synonym Gene Names
WIF-1

Uniprot Description

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

Research Articles on Wif1

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Product Notes

The Wif1 wif1 (Catalog #AAA206203) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Wnt inhibitory factor 1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Wif1 wif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GQPPEESLYL WIDAHQARVL IGFEEDILIV SEGKMAPFTH DFRKAQQRMP AIPVNIHSMN FTWQAAGQAE YFYEFLSLRS LDKGIMADPT VNVPLLGTVP HKASVVQVGF PCLGKQDGVA AFEVNVIVMN SEGNTILRTP QNAIFFKTCQ QAECPGGCRN GGFCNERRVC ECPDGFYGPH CEKALCIPRC MNGGLCVTPG FCICPPGFYG VNCDKANCST TCFNGGTCFY PGKCICPPGL EGEQCELSKC PQPCRNGGKC IGKSKCKCPK GYQGDLCSKP VCEPGCGAHG TCHEPNKCQC REGWHGRHCN KRYGASLMHA PRPAGAGLER HTPSLKKAED RRDPPESNYI WVEHHHHHH. It is sometimes possible for the material contained within the vial of "Wnt inhibitory factor 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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