Protein wingless (wg) Recombinant Protein | wg recombinant protein
Recombinant Drosophila melanogaster Protein wingless (wg)
NCBI and Uniprot Product Information
Uniprot Description
Function: Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. Segment polarity protein. May be a growth factor. Acts on neighboring cells to regulate at least one gene, the homeobox segmentation gene engrailed. Wg signal represses arm phosphorylation. Wg signaling operates by inactivating the sgg repression of engrailed autoactivation. Wg and Wnt2 have a role in the developing trachea and together are responsible for all dorsal trunk formation. Wg also acts in the developing epidermis. Acts as a morphogen, and diffuses long distances despite its lipidation. Lipophorin is required for diffusion, probably by acting as vehicle for its movement, explaining how it can spread over long distances despite its lipidation. In non-neuronal cells, wls directs wg secretion via clathrin-mediated endocytosis and the retromer complex (a conserved protein complex consisting of Vps26 and Vps35) to sustain a wls traffic loop encompassing the Golgi, the cell surface, an endocytic compartment and a retrograde route leading back to the Golgi. In neuronal cells (the larval motorneuron NMJ), wg signal moves across the synapse through the release of wls-containing exosome-like vesicles. Ref.11 Ref.15 Ref.16 Ref.17
Subunit structure: Monomer; folds by intramolecular disulfide bonds. Interacts with porcupine (por). Interacts with wls; in the Golgi. Ref.8 Ref.12 Ref.15
Subcellular location: Secreted. Cell junction › synapse. Membrane; Lipid-anchor. Secreted › extracellular space › extracellular matrix. Note: Palmitoylation converts wg into a membrane-anchored protein that is partitioned into specialized lipid raft microdomains before secretion. Possibly associated with the extracellular matrix. Ref.3 Ref.13 Ref.15 Ref.17
Tissue specificity: Segmented expression in embryos. In embryonic tracheal cells, expression is in stripes flanking the tracheal placode. Ref.1 Ref.11
Developmental stage: Expressed throughout development, but barely detectable in adults. Ref.1 Ref.2
Post-translational modification: Palmitoylated by porcupine, acylation is a prerequisite. The lipid group functions as a sorting signal, targeting the ligand to polarized vesicles that transport wg to unique sites at the cell surface. Major form is glycosylated at 2 sites, glycosylation is stimulated by porcupine at the ER, again acylation is a prerequisite. Ref.12
Disruption phenotype: Accumulation in the Golgi, thereby preventing secretion. Ref.16
Sequence similarities: Belongs to the Wnt family.
Research Articles on wg
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Product Notes
The wg wg (Catalog #AAA1148052) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 18-468aa; full length protein. The amino acid sequence is listed below: GSSLSQVEGK QKSGRGRGSM WWGIAKVGEP NNITPIMYMD PAIHSTLRRK QRRLVRDNPG VLGALVKGAN LAISECQHQF RNRRWNCSTR NFSRGKNLFG KIVDRGCRET SFIYAITSAA VTHSIARACS EGTIESCTCD YSHQSRSPQA NHQAGSVAGV RDWEWGGCSD NIGFGFKFSR EFVDTGERGR NLREKMNLHN NEAGRAHVQA EMRQECKCHG MSGSCTVKTC WMRLANFRVI GDNLKARFDG ATRVQVTNSL RATNALAPVS PNAAGSNSVG SNGLIIPQSG LVYGEEEERM LNDHMPDILL ENSHPISKIH HPNMPSPNSL PQAGQRGGRN GRRQGRKHNR YHFQLNPHNP EHKPPGSKDL VYLEPSPSFC EKNLRQGILG THGRQCNETS LGVDGCGLMC CGRGYRRDEV VVVERCACTF HWCCEVKCKL CRTKKVIYTC L. It is sometimes possible for the material contained within the vial of "Protein wingless (wg), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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