Platelet Derived Growth Factor A (PDGF-A) Western Blot Control | PDGF-A western blot control

Platelet Derived Growth Factor A (PDGF-A) (Western Blot Control)

Cat. Num. AAA343163

• Gene Names: PDGFA; PDGF1; PDGF-A
• Purity: >= 95% (SDS PAGE)
• Synonyms: Platelet Derived Growth Factor A (PDGF-A); Platelet Derived Growth Factor A (PDGF-A) (Western Blot Control); PDGF-A; Platelet derived growth factor A; PDGF1; platelet derived growth factor alpha.; PDGF-A western blot control

• Purity/Purification: >= 95% (SDS PAGE)
• Form/Format: Liquid
• Sequence Length: 196

• Buffer: Buffered 50% glycerol

Related Product Information for PDGF-A western blot control

The term PDGF refers to a family of disulphide bondlinked dimeric isoforms that act as autocrine and paracrine growth factors and are produced by a variety of cell types other than platelets. They act as potent mitogens for almost all mesenchymally-derived cells. Aberrant expression is involved in certain cancers, fibroproliferative disorders and atherosclerosis. The protein also contributes to wound healing and neural regeneration. There are four members of the PDGF family; PDGF A, PDGF B, PDGF C and PDGF D. Two distinct types of PDGF-A exist; a short form that is soluble and a long form that is retained by the extracellular matrix. Recombinant PDGF-A comprises a 110 amino acid fragment (87-196) corresponding to the mature PDGF-A short chain protein and is expressed in E Coli with an amino-terminal hexahistidine tag. This product is not intended for therapeutic use

Product Categories/Family for PDGF-A western blot control

Recombinant Proteins; Platelet Derived Growth Factor A Chain

References

Raines, E.W. & Ross, R. (1982) J. Biol. Chem. 257(9): 5154-5160. Bonthron, D.T. et al. (1988). Proc. Natl. Acad. Sci USA 85(5): 1492-1496.

NCBI and Uniprot Product Information

• NCBI GI #: 15208656
• NCBI GeneID: 5154
• NCBI Accession #: NP_148983.1
• NCBI GenBank Nucleotide #: NM_033023.4
• UniProt Accession #: P04085; B5BU73
• Molecular Weight: 22,253 Da
• NCBI Official Full Name: platelet-derived growth factor subunit A isoform 2 preproprotein
• NCBI Official Synonym Full Names: platelet derived growth factor subunit A
• NCBI Official Symbol: PDGFA
• NCBI Official Synonym Symbols: PDGF1; PDGF-A
• NCBI Protein Information: platelet-derived growth factor subunit A
• UniProt Protein Name: Platelet-derived growth factor subunit A
• UniProt Gene Name: PDGFA
• UniProt Synonym Gene Names: PDGF1; PDGF subunit A
• UniProt Entry Name: PDGFA_HUMAN

NCBI Description

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Cell cycle regulation; Secreted

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cell surface; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi membrane; microvillus

Molecular Function: collagen binding; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: actin cytoskeleton organization and biogenesis; alveolus development; angiogenesis; cell activation; cell projection biogenesis; cell-cell signaling; extracellular matrix organization and biogenesis; hair follicle development; MAPKKK cascade; negative chemotaxis; negative regulation of phosphatidylinositol biosynthetic process; organ morphogenesis; phosphoinositide-mediated signaling; platelet degranulation; platelet-derived growth factor receptor signaling pathway; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of protein kinase B signaling cascade; regulation of actin cytoskeleton organization and biogenesis; regulation of peptidyl-tyrosine phosphorylation; regulation of phosphoinositide 3-kinase cascade; regulation of smooth muscle cell migration; response to wounding; skin development; wound healing