Methylosome protein 50 Recombinant Protein | MEP50 recombinant protein

Recombinant Human Methylosome protein 50

Cat. Num. AAA9421364

• Gene Names: WDR77; p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Methylosome protein 50; Recombinant Human Methylosome protein 50; Androgen receptor cofactor p44WD repeat-containing protein 77p44/Mep50; MEP50 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Partial, 1-312aa

• Tag Info: His-SUMO-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for MEP50 recombinant protein

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assbly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

NCBI and Uniprot Product Information

• NCBI GI #: 951233290
• NCBI GeneID: 79084
• NCBI Accession #: NP_001303991.1
• NCBI GenBank Nucleotide #: NM_001317062.1
• UniProt Accession #: Q9BQA1; Q3LID2; Q53FU2; Q6JZZ5; Q96GK4; Q9BWY3; B3KMW6; B4DP38
• Molecular Weight: 49.5kD
• NCBI Official Full Name: methylosome protein 50 isoform 1
• NCBI Official Synonym Full Names: WD repeat domain 77
• NCBI Official Symbol: WDR77
• NCBI Official Synonym Symbols: p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50
• NCBI Protein Information: methylosome protein 50
• UniProt Protein Name: Methylosome protein 50
• Protein Family: Methylosome protein
• UniProt Gene Name: WDR77
• UniProt Synonym Gene Names: MEP50; WD45; MEP-50

NCBI Description

The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

WDR77: a WD40 repeat protein. Component of the methylosome, a 20S complex containing at least ICLN, SKB1 and WDR77. Interacts with SKB1 and with SM proteins. The methylosome may regulate an early step in the assembly of U snRNPs, possibly the transfer of Sm proteins to the SMN-complex. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.

Protein type: Adaptor/scaffold; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: cytoplasm; cytosol; Golgi apparatus; nucleoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor transcription coactivator activity; methyl-CpG binding; protein binding; protein-arginine N-methyltransferase activity

Biological Process: spliceosomal snRNP biogenesis