Mouse WD repeat-containing protein 35 ELISA Kit | WDR35 elisa kit
Mouse WD repeat-containing protein 35 (WDR35) ELISA Kit
Gene Names
Wdr35; mKIAA1336; 4931430C06; 4930459M12Rik
Reactivity
Mouse
Synonyms
WD repeat-containing protein 35; Mouse WD repeat-containing protein 35 (WDR35) ELISA Kit; WDR35 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse VLDLR. No significant cross-reactivity or interference between Mouse VLDLR and analogues was observed.
Sequence Length
1170
Samples
Serum, Plasma And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.31 ng/mL-20 ng/mL
Sensitivity
0.1 ng/mL (mean of 6 independent assays).
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for WDR35 elisa kit
Intended Uses: For the quantitative detection of Mouse Very low density lipoprotein receptor (VLDLR) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate VLDLR in Mouse serum, plasma. An antibody specific for VLDLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VLDLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VLDLR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VLDLR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate VLDLR in Mouse serum, plasma. An antibody specific for VLDLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VLDLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VLDLR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VLDLR bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
132,752 Da
NCBI Official Full Name
WD repeat-containing protein 35 isoform 2
NCBI Official Synonym Full Names
WD repeat domain 35
NCBI Official Symbol
Wdr35
NCBI Official Synonym Symbols
mKIAA1336; 4931430C06; 4930459M12Rik
NCBI Protein Information
WD repeat-containing protein 35
UniProt Protein Name
WD repeat-containing protein 35
Protein Family
UniProt Gene Name
Wdr35
Uniprot Description
WDR35: a WD40 repeat protein. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. Two alternatively spliced human isoforms have been described.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12|12 A1.1
Cellular Component: axoneme; cell projection; centrosome; cilium; cytoplasm; cytoskeleton
Molecular Function: phosphatidylinositol binding
Biological Process: cell projection organization; negative regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
Research Articles on WDR35
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Product Notes
The Mouse WDR35 wdr35 (Catalog #AAA280146) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280146 ELISA Kit recognizes Mouse WDR35. It is sometimes possible for the material contained within the vial of "WD repeat-containing protein 35, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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