Tryptophanyl tRNA Synthetase 2 Recombinant Protein | WARS2 recombinant protein

Recombinant Tryptophanyl tRNA Synthetase 2, Mitochondrial (WARS2)

Cat. Num. AAA2034621

• Gene Names: WARS2; TrpRS
• Reactivity: Mouse
• Applications: ELISA, Immunoprecipitation, SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Tryptophanyl tRNA Synthetase 2; Recombinant Tryptophanyl tRNA Synthetase 2; Mitochondrial (WARS2); Tryptophan tRNA Ligase 2; Mitochondrial; Tryptophanyl-tRNA synthetase; WARS2 recombinant protein

• Host: E Coli; Source: Prokaryotic expression
• Reactivity: Mouse
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 360

• Applicable Applications for WARS2 recombinant protein: SDS-PAGE; Western Blot (WB); ELISA (EIA); Immunoprecipitation (IP)
• Usage: Reconstitute in PBS or others.
• Endotoxin Level: < 1.0EU per 1ug (determined by the LAL method)
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Fragment: Met1~Leu360
• Tag: Two N-terminal Tags, His-tag and T7-tag
• Residues & Tags: Met1~Leu360 with N-terminal His Tag
• Subcellular Location: Secreted
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119577097
• NCBI GeneID: 10352
• Molecular Weight: Predicted: 43.9kDa
• NCBI Official Full Name: tryptophanyl tRNA synthetase 2 (mitochondrial), isoform CRA_a
• NCBI Official Synonym Full Names: tryptophanyl tRNA synthetase 2, mitochondrial
• NCBI Official Symbol: WARS2
• NCBI Official Synonym Symbols: TrpRS
• NCBI Protein Information: tryptophan--tRNA ligase, mitochondrial
• UniProt Protein Name: Tryptophan--tRNA ligase, mitochondrial
• UniProt Gene Name: WARS2
• UniProt Synonym Gene Names: TrpRS
• UniProt Entry Name: SYWM_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

WARS2: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Protein type: EC 6.1.1.2; Translation; Amino Acid Metabolism - tryptophan; Mitochondrial; Ligase

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: mitochondrial matrix

Molecular Function: tryptophan-tRNA ligase activity

Biological Process: tRNA aminoacylation for protein translation