WARS recombinant protein

Recombinant Human WARS Protein

Cat. Num. AAA8250023

• Gene Names: WARS; IFI53; IFP53; GAMMA-2
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: WARS; Recombinant Human WARS Protein; IFI53; WRS; Tryptophan--tRNA ligase cytoplasmic; Interferon-induced protein 53; IFP53; Tryptophanyl-tRNA synthetase; TrpRS; hWRS; WARS recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
• Sequence: MGSSHHHHHH SSGLVPRGSH MPNSEPASLL ELFNSIATQG ELVRSLKAGN ASKDEIDSAV KMLVSLKMSY KAAAGEDYKA DCPPGNPAPT SNHGPDATEA EEDFVDPWTV QTSSAKGIDY DKLIVRFGSS KIDKELINRI ERATGQRPHH FLRRGIFFSH RDMNQVLDAY ENKKPFYLYT GRGPSSEAMH VGHLIPFIFT KWLQDVFNVP LVIQMTDDEK YLWKDLTLDQ AYSYAVENAK DIIACGFDIN KTFIFSDLDY MGMSSGFYKN VVKIQKHVTF NQVKGIFGFT DSDCIGKISF PAIQAAPSFS NSFPQIFRDR TDIQCLIPCA IDQDPYFRMT RDVAPRIGYP KPALLHSTFF PALQGAQTKM SASDPNSSIF LTDTAKQIKT KVNKHAFSGG RDTIEEHRQF GGNCDVDVSF MYLTFFLEDD DKLEQIRKDY TSGAMLTGEL KKALIEVLQP LIAEHQARRK EVTDEIVKEF MTPRKLSFDF Q
• Sequence Length: 471

• Applicable Applications for WARS recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for WARS recombinant protein

Recombinant Human WARS Protein is produced by our E Coli expression system and the target gene encoding Met1-Gln471 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 47419914
• NCBI GeneID: 7453
• NCBI Accession #: NP_004175.2
• NCBI GenBank Nucleotide #: NM_004184.3
• UniProt Accession #: P23381; P78535; Q502Y0; Q53XB6; Q9UDI5; Q9UDL3; A6NGN1; A6NID3
• Molecular Weight: 48,852 Da
• NCBI Official Full Name: tryptophan--tRNA ligase, cytoplasmic isoform a
• NCBI Official Synonym Full Names: tryptophanyl-tRNA synthetase
• NCBI Official Symbol: WARS
• NCBI Official Synonym Symbols: IFI53; IFP53; GAMMA-2
• NCBI Protein Information: tryptophan--tRNA ligase, cytoplasmic
• UniProt Protein Name: Tryptophan--tRNA ligase, cytoplasmic
• Protein Family: Tryptophan--tRNA ligase
• UniProt Gene Name: WARS
• UniProt Synonym Gene Names: IFI53; WRS; IFP53; TrpRS; hWRS
• UniProt Entry Name: SYWC_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

WARS: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; EC 6.1.1.2; Ligase; Translation

Chromosomal Location of Human Ortholog: 14q32.31

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; tryptophan-tRNA ligase activity

Biological Process: negative regulation of cell proliferation; regulation of angiogenesis; translation; tRNA aminoacylation for protein translation