Porcine Von Willebrand Factor ELISA Kit | Vwf elisa kit

Porcine Von Willebrand Factor ELISA Kit

Cat. Num. AAA742459

• Gene Names: Vwf; VWD; F8VWF; AI551257; C630030D09; 6820430P06Rik; B130011O06Rik
• Reactivity: Porcine
• Synonyms: Von Willebrand Factor; Porcine Von Willebrand Factor ELISA Kit; Vwf elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of vWF. No significant cross-reactivity or interference between vWF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between vWF and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for Vwf elisa kit

Intended Uses: This vWF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine vWF. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: vWF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-vWF antibody and an vWF-HRP conjugate. The assay sample and buffer are incubated together with vWF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the vWF concentration since vWF from samples and vWF-HRP conjugate compete for the anti-vWF antibody binding site. Since the number of sites is limited, as more sites are occupied by vWF from the sample, fewer sites are left to bind vWF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The vWF concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Vwf elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 86129842
• NCBI GeneID: 22371
• Molecular Weight: 44,467 Da
• NCBI Official Full Name: VWF
• NCBI Official Synonym Full Names: Von Willebrand factor homolog
• NCBI Official Symbol: Vwf
• NCBI Official Synonym Symbols: VWD; F8VWF; AI551257; C630030D09; 6820430P06Rik; B130011O06Rik
• NCBI Protein Information: von Willebrand factor
• UniProt Protein Name: von Willebrand factor
• Protein Family: von Willebrand factor
• UniProt Gene Name: Vwf
• UniProt Synonym Gene Names: vWF
• UniProt Entry Name: VWF_MOUSE

Uniprot Description

VWF: Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Defects in VWF are the cause of von Willebrand disease type 1 (VWD1). A common hemorrhagic disorder due to defects in von Willebrand factor protein and resulting in impaired platelet aggregation. Von Willebrand disease type 1 is characterized by partial quantitative deficiency of circulating von Willebrand factor, that is otherwise structurally and functionally normal. Clinical manifestations are mucocutaneous bleeding, such as epistaxis and menorrhagia, and prolonged bleeding after surgery or trauma. Defects in VWF are the cause of von Willebrand disease type 2 (VWD2). A hemorrhagic disorder due to defects in von Willebrand factor protein and resulting in impaired platelet aggregation. Von Willebrand disease type 2 is characterized by qualitative deficiency and functional anomalies of von Willebrand factor. It is divided in different subtypes including 2A, 2B, 2M and 2N (Normandy variant). The mutant VWF protein in types 2A, 2B and 2M are defective in their platelet- dependent function, whereas the mutant protein in type 2N is defective in its ability to bind factor VIII. Clinical manifestations are mucocutaneous bleeding, such as epistaxis and menorrhagia, and prolonged bleeding after surgery or trauma. Defects in VWF are the cause of von Willebrand disease type 3 (VWD3). A severe hemorrhagic disorder due to a total or near total absence of von Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. Bleeding usually starts in infancy and can include epistaxis, recurrent mucocutaneous bleeding, excessive bleeding after minor trauma, and hemarthroses.

Protein type: Secreted, signal peptide; Cell adhesion; Secreted; Motility/polarity/chemotaxis; Extracellular matrix

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; endoplasmic reticulum; extracellular region; external side of plasma membrane

Molecular Function: collagen binding; integrin binding; identical protein binding; protein binding; protein homodimerization activity; protease binding; chaperone binding; protein N-terminus binding; immunoglobulin binding; glycoprotein binding

Biological Process: platelet activation; hemostasis; liver development; cell adhesion; blood coagulation; protein homooligomerization; cell-substrate adhesion; placenta development