Testing Data
(Fn-/- cells (1.25x10e5 cell/cm2) were seeded on wells coated with increasing concentrations of V2200-09B. Cells were allowed to attach for 20 minutes and then washed, fixed and stained with crystal violet. Data presented as the average|absorbance at 590nm of triplicate wells.)
Vitronectin Recombinant Protein | VTN recombinant protein
Vitronectin, Recombinant, Human
80% as shown by SDS-PAGE and Coomassie Blue staining.
80% as shown by SDS-PAGE and Coomassie Blue staining.
Dilution: Cell Adhesion Assay: Fn-/- cells (1.25x10e5 cell/cm2) were seeded on wells coated with increasing concentrations of V2200-09B. Cells were allowed to attach for 20 minutes and then washed, fixed and stained with crystal violet. Data presented as the average
absorbance at 590nm of triplicate wells.
Testing Data
(Fn-/- cells (1.25x10e5 cell/cm2) were seeded on wells coated with increasing concentrations of V2200-09B. Cells were allowed to attach for 20 minutes and then washed, fixed and stained with crystal violet. Data presented as the average|absorbance at 590nm of triplicate wells.)
Testing Data
(Fn-/- cells (1.25x10e5 cell/cm2) were seeded on wells coated with increasing concentrations of V2200-09B. Cells were allowed to attach for 20 minutes and then washed, fixed and stained with crystal violet. Data presented as the average|absorbance at 590nm of triplicate wells.)
SDS-Page
(SDS-PAGE and Coomassie Stain: Purity was assessed by SDS-PAGE and Coomassie blue staining using 5ug of|V2200-09B, (78kD).)
SDS-Page
(SDS-PAGE and Coomassie Stain: Purity was assessed by SDS-PAGE and Coomassie blue staining using 5ug of|V2200-09B, (78kD).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]
Uniprot Description
VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Cell adhesion; Secreted
Chromosomal Location of Human Ortholog: 17q11
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region
Molecular Function: integrin binding; heparin binding; protein binding; extracellular matrix binding; scavenger receptor activity; polysaccharide binding
Biological Process: receptor-mediated endocytosis; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell-matrix adhesion; negative regulation of blood coagulation; positive regulation of smooth muscle cell migration; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of receptor-mediated endocytosis; positive regulation of peptidyl-tyrosine phosphorylation; regulation of complement activation; cell adhesion mediated by integrin; innate immune response; immune response; cell adhesion
Research Articles on VTN
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Product Notes
The VTN vtn (Catalog #AAA638271) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Vitronectin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA and Cell Adhesion Assay. Dilution: Cell Adhesion Assay: Fn-/- cells (1.25x10e5 cell/cm2) were seeded on wells coated with increasing concentrations of V2200-09B. Cells were allowed to attach for 20 minutes and then washed, fixed and stained with crystal violet. Data presented as the average absorbance at 590nm of triplicate wells. Researchers should empirically determine the suitability of the VTN vtn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vitronectin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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